TCID | Name | Domain | Kingdom/Phylum | Protein(s) |
---|---|---|---|---|
1.E.4.1.1 | LydA protein | Bacteria |
Pseudomonadota | LydA protein (109 aas; pirS18681) |
1.E.4.1.2 | LydC of enterobacterial phage P1 | Viruses |
Heunggongvirae, Uroviricota | LydC of phage P1 |
1.E.4.1.3 | Hypothetical protein (113 aas) | Bacteria |
Pseudomonadota | Hypothetical protein of Burkholderia phytofirmans |
1.E.4.1.4 | Holin of 134 aas and 4 TMSs. The lysis genes encode the holin, peptidase M15A or endolysin, lysB and lysC. Each gene and combinations were cloned into Escherichia coli and the lytic effects were measured. Co-expression of holin and peptidase M15A showed the highest lysis activity. Expression of holin, lysB/C or holin, peptidase M15A, lysB and lysC lysed the E. coli membrane whereas peptidase M15A alone did not. The predicted transmembrane structures of holin and lysB/C indicated they could insert into the bacterial membrane to form pores, affecting cell permeability and causing lysis (Khakhum et al. 2016). | Viruses |
Heunggongvirae, Uroviricota | Holin of Burkholderia phage ST79 |