2.A.104.1.1
The L-alanine exporter, AlaE (YgaW) of 149 aas and 4 TMSs. (In the DUF1144 or PF06610 superfamily) (Hori et al., 2011). Two charged residues are essential for it's efflux activity (Kim et al. 2016). AlaE is the physiologically most relevant exporter for L-alanine in E. coli.
AlaE forms homo-oligomers, and the GxxxG motif in the TMS4 region plays
an essential role in AlaE activity but not in AlaE oligomer formation (Ihara et al. 2022).
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| Accession Number: | A8ANM6 |
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| Protein Name: | L-alanine exporter AlaE |
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| Length: | 149 |
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| Molecular Weight: | 16685.00 |
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| Species: | Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [290338] |
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| Number of TMSs: | 4 |
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| Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 |
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| Substrate |
alanine |
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1: MFSPQSRLRH AVADTFAMVV YCSVVNMLIE IFLSGMTFEQ SLSSRLVAIP VNILIAWPYG
61: MYRDAIMRVA RKASPASWVK NLADVLAYVT FQSPVYVAIL LTVGADWHQI TAAVSSNIVI
121: SMLMGAVYGY FLDYCRRLFK VSSYHQAKA