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2.A.19.2.7
Ca2+/H+ antiporter, YfkE (Fujisawa et al., 2009). YfkE is a homotrimer with a subunit size of 451 aas.  The 3-d x-ray strcuture is known to 3.1 Å resolution (Wu et al. 2013).  The conformational transition is triggered by the rotation of the kink angles of transmembrane helices 2 and 7 and is mediated by large conformational changes in their adjacent transmembrane helices 1 and 6.  The inward facing conformation contrasts with the outward facing conformation demonstrated for NCX_Mj (TC# 2.A.19.5.3).  The inward facing conformation has a "hydrophobic seal" that closes the external exit (Wu et al. 2013). Intracellular Ca2+ regulation of this H+/Ca2+ antiporter is mediated by a Ca2+ mini-sensor (Lu et al. 2020).

Accession Number:O34840
Protein Name:Putative cation exchanger yfkE
Length:351
Molecular Weight:37522.00
Species:Bacillus subtilis [1423]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Ca2+, H+

Cross database links:

RefSeq: NP_388673.1   
Entrez Gene ID: 939693   
Pfam: PF01699   
BioCyc: SUBTI:BSU07920-MONOMER   
KEGG: bsu:BSU07920   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008324 F:cation transmembrane transporter activity
GO:0006812 P:cation transport
GO:0055085 P:transmembrane transport

References (2)

[1] “Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees region of the Bacillus subtilis chromosome containing genes for trehalose metabolism and acetoin utilization.”  Yamamoto H.et.al.   8969503
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
Structure:
4KJR   4KJS     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNRIFFILVA AGVPLSVIGS LMHWPSAVLF AVYCVTIIAL ASYMGRATES LSIIAGPRIG 
61:	GLLNATFGNA VELIISLFAL KEGLTGIVLA SLTGSVLGNL LLVAGLSFFV GGLKYKRQEF 
121:	NIHDARHNSG LLIFAIIVAF VIPEVFSVGM GNASKLNLSI GISIIMILLY VAALYFKLVT 
181:	HRGVYQPNNA AQTEEEEEPE WSGKVATIVL FAATIVVAYI SENLVHTFHS VAEQFGWSEL 
241:	FIGVIIVAIV GNAAEHASAI IMAFKNKMDI AVEIAVGSTL QIAMFVAPVL VICSIFFPTS 
301:	MPLVFTLPEL VAMVSAVLLM IAISNDGDSN WFEGATLLAA YVIMAIGFFL L