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2.A.31.2.3
Electrogenic Na+:HCO3- symporter/Cl- antiporter, NCBE (regulates intracellular pH) (Wang et al., 2000; Damkier et al., 2010). Expressed in specific brain cell types; glycosylation required for functional expression (Chen et al., 2008). Loss reduces brain ventricle volume, impairs visual function and protects against fatal epileptic seizures in mice (Hilgen et al. 2012).  NCBE is involved in the control of neuronal pH and excitability; may contribute to the secretion of cerebrospinal fluid (Jacobs et al., 2008). The human orthologue (Q6U841) is an electroneutral Na+/HCO3- cotransporter (NBCn2 or NCBE) with Cl- self exchange activity (Parker et al., 2008).  NCBE/NBCn2 is predominantly expressed in the central nervous system (CNS) with highest concentrations in the choroid plexus. Its primary function is to regulate intracellular neuronal pH and to maintain the pH homeostasis across the blood-cerebrospinal fluid barrier. NCBE is predicted to contain at least 10 transmembrane helices. The N- and C- termini are both cytoplasmic, with a large N-terminal domain (Nt-NCBE) and a relatively small C-terminal domain (Ct-NCBE). The Nt-NCBE is likely to be involved in bicarbonate recognition and transport and contains key areas of regulation involving pH sensing and protein-protein interactions.  It has an intrinsic disordered structure (Bjerregaard-Andersen et al. 2013).

Accession Number:Q5DTL9
Protein Name:Sodium-driven chloride bicarbonate exchanger
Length:1118
Molecular Weight:125817.00
Species: [10090]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Na+, bicarbonate, Cl-

Cross database links:

RefSeq: NP_291030.2   
Entrez Gene ID: 94229   
Pfam: PF07565    PF00955   
KEGG: mmu:94229   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0005452 F:inorganic anion exchanger activity
GO:0015293 F:symporter activity
GO:0006820 P:anion transport
GO:0006885 P:regulation of pH
GO:0006814 P:sodium ion transport

References (5)

[1] “The Na+-driven Cl-/HCO3- exchanger. Cloning, tissue distribution, and functional characterization.”  Wang C.-Z.et.al.   10993873
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “A SCL4A10 gene product maps selectively to the basolateral plasma membrane of choroid plexus epithelial cells.”  Praetorius J.et.al.   14592810
[4] “Comprehensive identification of phosphorylation sites in postsynaptic density preparations.”  Trinidad J.C.et.al.   16452087
[5] “Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.”  Munton R.P.et.al.   17114649

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEIKDQGAQM EPLLPTRNDE EAVVDRGGTR SILKTHFEKE DLEGHRTLFI GVHVPLGGRK 
61:	SHRRHRHRGH KHRKRDRERD SGLEDGRESP SFDTPSQRVQ FILGTEDDDE EHLPHDLFTE 
121:	LDEICWREGE DAEWRETARW LKFEEDVEDG GERWSKPYVA TLSLHSLFEL RSCILNGTVL 
181:	LDMHANTIEE IADMVLDQQV SSGQLNEDVR HRVHEALMKQ HHHQNQKKLA NRIPIVRSFA 
241:	DIGKKQSEPN SMDKNAGQVV SPQSAPACAE NKNDVSRENS TVDFSKGLGG QQKGHTSPCG 
301:	MKQRLDKGPP HQQEREVDLH FMKKIPPGAE ASNILVGELE FLDRTVVAFV RLSPAVLLQG 
361:	LAEVPIPSRF LFILLGPLGK GQQYHEIGRS IATLMTDEVF HDVAYKAKDR NDLVSGIDEF 
421:	LDQVTVLPPG EWDPSIRIEP PKNVPSQEKR KIPAVPNGTA AHGEAEPHGG HSGPELQRTG 
481:	RIFGGLILDI KRKAPFFWSD FRDAFSLQCL ASFLFLYCAC MSPVITFGGL LGEATEGRIS 
541:	AIESLFGASM TGIAYSLFGG QPLTILGSTG PVLVFEKILF KFCKEYGLSY LSLRASIGLW 
601:	TATLCIILVA TDASSLVCYI TRFTEEAFAS LICIIFIYEA LEKLFELSET YPINMHNDLE 
661:	LLTQYSCNCM EPHSPSNDTL KEWRESNLSA SDIIWGNLTV SECRSLHGEY VGRACGHGHP 
721:	YVPDVLFWSV ILFFSTVTMS ATLKQFKTSR YFPTKVRSIV SDFAVFLTIL CMVLIDYAIG 
781:	IPSPKLQVPS VFKPTRDDRG WFVTPLGPNP WWTIIAAIIP ALLCTILIFM DQQITAVIIN 
841:	RKEHKLKKGC GYHLDLLMVA VMLGVCSIMG LPWFVAATVL SITHVNSLKL ESECSAPGEQ 
901:	PKFLGIREQR VTGLMIFILM GSSVFMTSIL KFIPMPVLYG VFLYMGASSL KGIQLFDRIK 
961:	LFWMPAKHQP DFIYLRHVPL RKVHLFTVIQ MSCLGLLWII KVSRAAIVFP MMVLALVFVR 
1021:	KLMDFLFTKR ELSWLDDLMP ESKKKKLEDA EKEEEQSMLA MEDEGTVQLP LEGHYRDDPS 
1081:	VINISDEMSK TAMWGNLLVT ADNSKEKESR FPSKSSPS