Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.31.2.4
Electroneutral Na+-driven HCO3-/Cl- (+ H+) exchanger, NDCBE1; SLC4A8; NBCn1 (Boron et al. 2009). The crystal structure of the regulatory domain of the human sodium-driven chloride/bicarbonate exchanger  at 2.8 Å resolution has been determined (Alvadia et al. 2017).  It is essential for maintaining a homeostatic pH in neurons.  It forms an equivalent dimeric interface as observed for the cytoplasmic domain of Band 3, and thus establishes that the consensus motif VTVLP is the key minimal dimerization motif. It is highly conserved and likely to be the physiologically relevant interface for all other members of the AE family. A conserved Zn2+-binding motif present in the N-terminal domain of NDCBE was identified and characterized in vitro. Cellular studies confirmed the Zn2+-dependent transport of two electroneutral bicarbonate transporters, NCBE and NBCn1. The Zn2+ site was mapped to a cluster of histidines close to the conserved ETARWLKFEE motif and likely plays a role in the regulation of this motif (Alvadia et al. 2017). Lee et al. 2022 concluded that this system transporters Cl- and HCO3- in a Na+-dependent antiport process.

Accession Number:Q2Y0W8
Protein Name:Electroneutral sodium bicarbonate exchanger 1
Length:1093
Molecular Weight:122938.00
Species: [9606]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate chloride, sodium(1+), hydrogencarbonate

Cross database links:

RefSeq: NP_001035049.1    NP_004849.2   
Entrez Gene ID: 9498   
Pfam: PF07565    PF00955   
OMIM: 605024  gene
KEGG: hsa:9498    hsa:9498   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005452 F:inorganic anion exchanger activity
GO:0006820 P:anion transport
GO:0006814 P:sodium ion transport
GO:0005886 C:plasma membrane
GO:0015701 P:bicarbonate transport
GO:0055085 P:transmembrane transport

References (14)

[1] “Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger.”  Grichtchenko I.I.et.al.   11133997
[2] “Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.”  Nagase T.et.al.   9872452
[3] “The full-ORF clone resource of the German cDNA consortium.”  Bechtel S.et.al.   17974005
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “Characterization of Na+/HCO-3 cotransporter isoform NBC-3.”  Amlal H.et.al.   10362779
[6] “Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues.”  Damkier H.H.et.al.   17715183
[7] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[8] “Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger.”  Grichtchenko I.I.et.al.   11133997
[9] “Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.”  Nagase T.et.al.   9872452
[10] “The full-ORF clone resource of the German cDNA consortium.”  Bechtel S.et.al.   17974005
[11] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[12] “Characterization of Na+/HCO-3 cotransporter isoform NBC-3.”  Amlal H.et.al.   10362779
[13] “Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues.”  Damkier H.H.et.al.   17715183
[14] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
Structure:
5JHO     

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MPAAGSNEPD GVLSYQRPDE EAVVDQGGTS TILNIHYEKE ELEGHRTLYV GVRMPLGRQS 
61:	HRHHRTHGQK HRRRGRGKGA SQGEEGLEAL AHDTPSQRVQ FILGTEEDEE HVPHELFTEL 
121:	DEICMKEGED AEWKETARWL KFEEDVEDGG ERWSKPYVAT LSLHSLFELR SCLINGTVLL 
181:	DMHANSIEEI SDLILDQQEL SSDLNDSMRV KVREALLKKH HHQNEKKRNN LIPIVRSFAE 
241:	VGKKQSDPHL MDKHGQTVSP QSVPTTNLEV KNGVNCEHSP VDLSKVDLHF MKKIPTGAEA 
301:	SNVLVGEVDI LDRPIVAFVR LSPAVLLSGL TEVPIPTRFL FILLGPVGKG QQYHEIGRSM 
361:	ATIMTDEIFH DVAYKAKERD DLLAGIDEFL DQVTVLPPGE WDPSIRIEPP KNVPSQEKRK 
421:	MPGVPNGNVC HIEQEPHGGH SGPELQRTGR LFGGLVLDIK RKAPWYWSDY RDALSLQCLA 
481:	SFLFLYCACM SPVITFGGLL GEATEGRISA IESLFGASMT GIAYSLFAGQ ALTILGSTGP 
541:	VLVFEKILFK FCKDYALSYL SLRACIGLWT AFLCIVLVAT DASSLVCYIT RFTEEAFASL 
601:	ICIIFIYEAI EKLIHLAETY PIHMHSQLDH LSLYYCRCTL PENPNNHTLQ YWKDHNIVTA 
661:	EVHWANLTVS ECQEMHGEFM GSACGHHGPY TPDVLFWSCI LFFTTFILSS TLKTFKTSRY 
721:	FPTRVRSMVS DFAVFLTIFT MVIIDFLIGV PSPKLQVPSV FKPTRDDRGW IINPIGPNPW 
781:	WTVIAAIIPA LLCTILIFMD QQITAVIINR KEHKLKKGCG YHLDLLMVAI MLGVCSIMGL 
841:	PWFVAATVLS ITHVNSLKLE SECSAPGEQP KFLGIREQRV TGLMIFVLMG CSVFMTAILK 
901:	FIPMPVLYGV FLYMGVSSLQ GIQFFDRLKL FGMPAKHQPD FIYLRHVPLR KVHLFTLIQL 
961:	TCLVLLWVIK ASPAAIVFPM MVLALVFVRK VMDLCFSKRE LSWLDDLMPE SKKKKLDDAK 
1021:	KKAKEEEEAE KMLEIGGDKF PLESRKLLSS PGKNISCRCD PSEINISDEM PKTTVWKALS 
1081:	MNSGNAKEKS LFN