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2.A.37.1.4
K+ efflux antiporter 1, chloroplastic (AtKEA1).  It localizes to the chloroplast inner envelope membrane and is essential for normal osmoregulation (Kunz et al. 2014). It has an even number of TMSs, probably 14, with N- and C-terminal hydrophilic regulatory domains (Bölter et al. 2019). 

Accession Number:Q9ZTZ7
Protein Name:K(+) efflux antiporter 1, chloroplastic
Length:1193
Molecular Weight:128033.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Plastid1 / Multi-pass membrane protein2
Substrate H+, K+

Cross database links:

Genevestigator: Q9LQ76
eggNOG: COG1226
HEGENOM: HOG000100760
Entrez Gene ID: 837332   
Pfam: PF00999    PF02254   
KEGG: ath:AT1G01790   

Gene Ontology

GO:0031969 C:chloroplast membrane
GO:0016021 C:integral to membrane
GO:0000166 F:nucleotide binding
GO:0015299 F:solute:hydrogen antiporter activity
GO:0006813 P:potassium ion transport

References (7)

[1] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[2] “Phylogenetic relationships within cation transporter families of Arabidopsis.”  Maeser P.et.al.   11500563
[3] “Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis.”  Froehlich J.E.et.al.   12938931
[4] “Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana.”  Ferro M.et.al.   12766230
[5] “The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions.”  Kleffmann T.et.al.   15028209
[6] “Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.”  Zybailov B.et.al.   18431481
[7] “Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.”  Reiland S.et.al.   19376835

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MEYASTFQRP ILFHGGDGAS YCFPNRLISP KGISITSGDS KVHSCFRLRR NVAQSGTLNL 
61:	MNACFSGRFY SGHLHSTKSI LGNGHQAKRI PFGFRLRCQG HESLGNADSN DHRIGESSES 
121:	SDETEATDLK DARVENDTDS LEELKELLHK AIKELEVARL NSTMFEEKAQ RISERAIALK 
181:	DEAATAWLDV NKTLDVIRDT VYEEALAKEA VQTATMALSL AEARLQVIVE SLEAGAGNDI 
241:	PHVSEETEET IDVNDKEEAL LAAKDDIKEC QVNLDNCESQ LSALLSKKDE LQKEVDKLNE 
301:	FAETIQISSL KAEEDVTNIM KLAEQAVAFE LEATQRVNDA EIALQRAEKS LSISQTPEET 
361:	QGQLSDEETS QEDAMVLSGN VEDVTHQVEK ESPKDGDLPV VQITAELVPD IVGQRNKKLT 
421:	QPYESSDHEN GKPSVESSKV VEADSEKPKI NVQTKKQETQ KDLPKEGSSL NSPKASFNKS 
481:	SRFFSASFFS SNPDGTATVF GSLVGSVKQQ WPKLVLGLAL LGAGLTLYSN GVGGNNQLLQ 
541:	QPDVTSTSTE DVSSSTKPLI RQVQKLPKRI KKLLEMIPHQ EVNEEEASLF DFLWLLLASV 
601:	IFVPLFQKIP GGSPVLGYLA AGILIGPYGL SIIRNVHGTR AIAEFGVVFL LFNIGLELSV 
661:	ERLSSMKKYV FGLGSAQVLV TAAVVGLLAH YVAGQAGPAA IVIGNGLALS STAVVLQVLQ 
721:	ERGESTSRHG RASFSVLLFQ DLAVVVLLIL IPLISPNSSK GGIGFQAIAE ALGLAAVKAA 
781:	VAITAIIAGG RLLLRPIYKQ IAENRNAEIF SANTLLVILG TSLLTARAGL SMALGAFLAG 
841:	LLLAETEFSL QVESDIAPYR GLLLGLFFMT VGMSIDPKLL LSNFPVIVGT LGLLIVGKTM 
901:	LVVIMGKLFG ISIISAIRVG LLLAPGGEFA FVAFGEAVNQ GIMSPQLSSL LFLVVGISMA 
961:	ITPWLAAGGQ LIASRFELHD VRSLLPVESE TDDLQGHIII CGFGRVGQII AQLLSERLIP 
1021:	FVALDVSSDR VTIGRSLDLP VYFGDAGSKE VLHKIGAGRA CAAVVALDAP GANYRCVWAL 
1081:	SKFYPNVKTF VRAHDVVHGL NLEKAGATAV VPETLEPSLQ LAAAVLAQAK LPTSEIANTI 
1141:	NEFRTRHLSE LTELCEASGS SLGYGYSRTS KPKPQPSDAS GDNQIIEGGT VVI