2.A.39.3.13 Uracil-specific permease of 581 aas and 11 or 12 TMSs. FUR4. Deletion of the FUR4 gene confers resistance to 5-fluorouracil as well
as cross-resistance to triazoles and imidazole antifungal agents when they are
used simultaneously with 5-fluorouracil although the nucleobase
transporters are not involved in azole uptake. Only fluorinated
pyrimidines, not pyrimidines themselves, are able to promote
cross-resistance to azoles by both the salvage and the de novo pathway
of pyrimidine synthesis. Subinhibitory doses of 5-fluorocytosine,
5-fluorouracil, and 5-fluorouridine also trigger resistance
to fluconazole in susceptible wild-type strains of C. lusitaniae and of
different Candida species. Thus, intracellular
fluorinated nucleotides play a role in azole resistance, either by
preventing azoles from targeting the catalytic site of lanosterol 14-alpha-demethylase or by acting as a molecular switch for the triggering
of efflux transport (Gabriel et al. 2014; ).
|
Accession Number: | R9WAS9 |
Protein Name: | Uracil permease |
Length: | 581 |
Molecular Weight: | 64936.00 |
Species: | Clavispora lusitaniae [36911] |
Number of TMSs: | 11 |
Substrate |
uracil |
---|
1: MSSATKGYDD TFTKSESDSQ SVTSVVDEKP SLWQRINKKI SLQNPDLSLV QNFLINDDLK
61: PLLNPQDRTW RWFHFIFFWI SDSYNINTWQ IAGTGVEAGL SWWEVWISVI LGYSFVGLFI
121: FLAQRVGAYY HVSFPVSCRA SFGIFGSLWP IINRVVMAVV WASVQSWIGG QCVQLMLMSI
181: FGADLDTKIP NKIKGSGTTS FQFLCFFLFC FFSLPAIYLK PQKLRHLFTM KAIVSPACGI
241: ALLIWTIKRA GGIGPVVHQK TSVSGSTHAW AFINSTMNCI ANFATLTLNA PDFSRLAKTR
301: HSSSWTQAIS IPLSFALTAI IGILVSSASK VMYGTAYWSP LDVMKEYVMS YTSGDRGGVF
361: IIALGFAIAQ LGTNISANYI SAGTDMTALL PQYINIRRGG FIAAAICFTI CPWHFFSSSK
421: NFTTYLSAYA VFLSAITGVI ASDYFWVRRG YLNVWHLYSF TPESNYSYNS LGVNWRAYVA
481: YICGILPNIV GFVGACGVSI PIGAQYVYNL SYFAGYIVSF TIYALFCFFW PVKGAPVKDI
541: REKGWYEVYV EDRPGEKFSD IVSGKLEENE YVRARGGIRL L