TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
2.A.69.1.1









Auxin efflux carrier, PIN-FORMED1 (PIN1) (Reinhardt et al., 2003; Carraro et al. 2012). Catalyzes auxin efflux without the participation of any other protein (Petrasek et al., 2006).  PIN1 determines the direction of intercellular auxin flow (Wang et al. 2014). It consists of two TMS bundles, each of 5 TMSs at the N-terminus and the C-terminus of the protein (NodzyƄski et al. 2016), confirming previous bioinformatic predictions (Mansour et al. 2007). Arabidopsis VASCULATURE COMPLEXITY AND CONNECTIVITY (VCC) (TC# 8.A.175) is a plant-specific transmembrane protein that controls the development of veins in cotyledons. Yanagisawa et al. 2021 showed that the expression and localization of PIN1 is altered in vcc developing cotyledons, and that overexpression of PIN1-GFP partially rescues vascular defects of vcc mutants. Genetic analyses suggested that VCC and PINOID (PID), a kinase that regulates PIN1 polarity, are both required for PIN1-mediated control of vasculature development. VCC expression is upregulated by auxin, likely as part of a positive feedback loop for the progression of vascular development. VCC and PIN1 localized to the plasma membrane in pre-procambial cells but were actively redirected to vacuoles in procambial cells for degradation. In the vcc mutant, PIN1 failed to properly polarize in pre-procambial cells during the formation of basal strands, and instead, it was prematurely degraded in vacuoles. VCC plays a role in the localization and stability of PIN1, which is crucial for the transition of pre-procambial cells into procambial cells that are involved in the formation of basal lateral strands in embryonic cotyledons (Yanagisawa et al. 2021).

Eukaryota
Viridiplantae
PIN1 of Arabidopsis thaliana
2.A.69.1.2









Auxin transporter, ethylene-insensitive root 1 (EIR1) auxin:H+ symporter
Eukaryota
Viridiplantae
EIR1 of Arabidopsis thaliana
2.A.69.1.3









Auxin efflux carrier, PIN7 (promotes embryonic axis formation) (Friml et al., 2003)
Eukaryota
Viridiplantae
PIN7 of Arabidopsis thaliana (NP_849923)
2.A.69.1.4









Auxin efflux facilitator PIN3: functions in auxin redistribution through the root cap in response to the gravity sensors, ARL2 (Q6XL73) and ARG1 (Q9ZSY2). Both ARG1 and ARL2 are DnaJ homologues and show regions homologous to translocation proteins, NPL1 and Sec63 (3.A.5.8.1 and 3.A.5.9.1, respectively.)
Eukaryota
Viridiplantae
PIN3 of Arabidopsis thaliana
(Q9S7Z8).
2.A.69.1.5









Auxin efflux carrier #10, PIN10/SLM1 (important for development; orthologous to A. thaliana PIN1 (Zhou et al., 2011)).

Eukaryota
Viridiplantae
PIN10 of Medicago truncatula (Q673E5)
2.A.69.1.6









Putative auxin efflux carrier component 8 (AtPIN8)
Eukaryota
Viridiplantae
PIN8 of Arabidopsis thaliana
2.A.69.1.7









Auxin efflux carrier, PIN5.  Regulates auxin homeostasis and metabolism.  Mediates auxin transport across the endoplasmic reticular membrane, for the cytosol to the ER lumen (Mravec et al. 2009).

Eukaryota
Viridiplantae
PIN5 of Arabidopsis thaliana
2.A.69.1.8









The auxin efflux carrier, AEC3 or PIN1, of 634 aas and 10 TMSs, plays a role in fruit development (Li et al. 2017).

Eukaryota
Viridiplantae
AEC3 of Momordica charantia (bitter gourd)
2.A.69.2.1









AEC family member of 416 aas and 10 TMSs in a 5 + 5 TMS arrangement, with a large hydrophilic loop between the two repeat units.

Bacteria
Proteobacteria
AEC family member of Trypanosoma cruzi (E7LII7)
2.A.69.2.2









Poorly characterized transporter YBR287w.  Deletion of the gene leads to poor growth on glucose-minimal medium at 15 degrees C in the FY 1679 genetic background, but is not involved in mating or sporulation (Dueñas et al. 1999).  

Eukaryota
Fungi
YBR287w of Saccharomyces cerevisiae
2.A.69.2.3









Auxin efflux carrier family member

Eukaryota
Fungi
AEC homologue of Aspergillus flavus (B8MZ51)
2.A.69.2.4









AEC family member

Eukaryota
Entamoeba
AEC family member of Entamoeba histolytica (C4MAS5)
2.A.69.2.5









Uncharacterized protein of 616 aas and 11 TMSs in a 5 + 6 arrangement

Eukaryota
Bangiophyceae
UP of Cyanidioschyzon merolae
2.A.69.2.6









Uncharacterized transporter, nonessential for growth or sporulation, YLR152c, of 576 aas and 10 TMSs (Watson 2001).

Eukaryota
Fungi
UP of Saccharomyces cerevisiae
2.A.69.2.7









Uncharacterized protein of 442 aas and 10 TMSs in a 5 + 5 TMS arrangement.

Eukaryota
Viridiplantae
UP of Citrus clementina
2.A.69.3.1









AEC homologue

Bacteria
Actinobacteria
AEC homologue of Streptomyces coelicolor
2.A.69.3.2









Malate transporter (MleP) homologue

Bacteria
Proteobacteria
MleP homologue of Dickeya dadantii (E0SFK1)
2.A.69.3.3









Putative malonate transporter, MdcF
Bacteria
Proteobacteria
MdcF of Klebsiella pneumoniae
2.A.69.3.4









Putative malonate transporter, MdcF

Bacteria
Proteobacteria
MdcF of Rhizobium meliloti
2.A.69.3.5









Uncharacterized transporter YfdV
Bacteria
Proteobacteria
YfdV of Escherichia coli O6:H1
2.A.69.3.6









Putative MdcF malonate transporter of 316 aas and 10 TMSs.

Bacteria
Proteobacteria
MdcF homologue of Rhizobium loti
2.A.69.3.7









AEC family transporter, auxin efflux carrier, of 318 aas and 10 TMSs in a 5 + 5 TMS arrangement. The efflux mechanism of the substrate, indole 3-acetic acid, has been examined (Rai et al. 2021).

Bacteria
Terrabacteria group
AEC of Bacillus licheniformis
2.A.69.4.1









Putative membrane permease-like protein. May belong to the BART superfamily (297aas; 10TMS)

Bacteria
Bacteroidetes/Chlorobi group
permease-like protein of Chlorobium luteolum (Q3B5D8)
2.A.69.4.2









Uncharacterized transporter MJ1031
Archaea
Euryarchaeota
MJ1031 of Methanocaldococcus jannaschii
2.A.69.4.3









Uncharacterized transporter MTH_1382
Archaea
Euryarchaeota
MTH_1382 of Methanothermobacter thermautotrophicus
2.A.69.4.4









AEC homologue

Bacteria
Proteobacteria
AEC homologue of Myxococcus xanthus
2.A.69.4.5









Malate permease, MleP (Labarre et al. 1996). Activated a few minutes after rehydration (Costantini et al. 2011).

Bacteria
Firmicutes
MleP of Oenococcus (Leuconostoc) oeni
2.A.69.4.6









Putative auxin efflux carrier of 333 aas and 10 TMSs.

Bacteria
Actinobacteria
Auxin efflux carrier of Bifidobacterium longum
2.A.69.4.7









Uncharacterized protein of 308 aas and 10 TMSs (Hug et al. 2016).

Bacteria
Candidatus Peregrinibacteria
UP of Candidatus Peribacter riflensis
2.A.69.4.8









Uncharacterized protein of 388 aas and 10 TMSs in a 5 + 5 TMS arrangement.

Eukaryota
Entamoebidae
UP of Entamoeba histolytica