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3.A.1.118.1
The cyclic peptide antibiotic, microcin J25 (MccJ25; the precursor peptide is JcjA) exporter, the self immunity protein, McjD. TolC is also required for export; Vincent and Morero, 2009.  The 3-d structure has been determined to 2.7Å resolution in an outward occluded state (Choudhury et al. 2014).  Binding and efflux as well as stimulation of the ATPase activity upon binding of MccJ25 have been demonstrated (Choudhury et al. 2014).  This is one of two MCCJ25 exporters, the other being YojI (TC# 3.A.1.113.3).  The large conformational changes in some crystal structures may not be necessary even for a large substrate like MccJ25 (Gu et al. 2015).

Accession Number:Q9X2W0
Protein Name:McjD
Length:580
Molecular Weight:65425.00
Species:Escherichia coli [562]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

Pfam: PF00005   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0030153 P:bacteriocin immunity
GO:0043213 P:bacteriocin transport
GO:0055085 P:transmembrane transport

References (2)

[1] “Sequence analysis of the four plasmid genes required to produce the circular peptide antibiotic microcin J25.”  Solbiati J.O.et.al.   10198038
[2] “Genetic analysis of plasmid determinants for microcin J25 production and immunity.”  Solbiati J.O.et.al.   8655570
Structure:
4PL0   5EG1   5OFP   5OFR     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MERKQKNSLF NYIYSLMDVR GKFLFFSMLF ITSLSSIIIS ISPLILAKIT DLLSGSLSNF 
61:	SYEYLVLLAC LYMFCVISNK ASVFLFMILQ SSLRINMQKK MSLKYLRELY NENITNLSKN 
121:	NAGYTTQSLN QASNDIYILV RNVSQNILSP VIQLISTIVV VLSTKDWFSA GVFFLYILVF 
181:	VIFNTRLTGS LASLRKHSMD ITLNSYSLLS DTVDNMIAAK KNNALRLISE RYEDALTQEN 
241:	NAQKKYWLLS SKVLLLNSLL AVILFGSVFI YNILGVLNGV VSIGHFIMIT SYIILLSTPV 
301:	ENIGALLSEI RQSMSSLAGF IQRHAENKAT SPSIPFLNME RKLNLSIREL SFSYSDDKKI 
361:	LNSVSLDLFT GKMYSLTGPS GSGKSTLVKI ISGYYKNYFG DIYLNDISLR NISDEDLNDA 
421:	IYYLTQDDYI FMDTLRFNLR LANYDASENE IFKVLKLANL SVVNNEPVSL DTHLINRGNN 
481:	YSGGQKQRIS LARLFLRKPA IIIIDEATSA LDYINESEIL SSIRTHFPDA LIINISHRIN 
541:	LLECSDCVYV LNEGNIVASG HFRDLMVSNE YISGLASVTE