3.A.29.1.7
ATP-dependent zinc metalloprotease, FtsH, of 610 aas and 2 TMSs. It acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins, and it plays a role in the quality control of integral membrane proteins.The cryo-EM structure in a fully ADP-bound symmetric state has been solved (Liu et al. 2022). Comparisons of the ADP-state structure with its apo-state and a substrate-engaged yeast YME1 structure show conformational changes in the ATPase domains, rather than the protease domains. A reconstruction of the full-length TmFtsH provides structural insights for the dynamic transmembrane and the periplasmic domains. The structural analyses expand the understanding of conformational switches between different nucleotide states in ATP hydrolysis by FtsH (Liu et al. 2022).