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3.A.3.2.19
Endomembrane (Golgi) Ca2+/Mn2+-ATPase, ECA3 (one of 4 close paralogues in A. thaliana (Mills et al., 2008; Kamrul Huda et al. 2013). It has 998 aas and 10 TMSs in a 2 + 2 + 6 TMS arrangement. ECA3 localizes to the trans-Golgi apparatus and plays a role in response to Mn2+ deficiency with severe effects seen in an eca3 nramp1 (see TC# 2.A.55.2.4) nramp2 triple mutant under low Mn2+ supply. ECA3 plays a minor role in Mn-toxicity tolerance, but only when the cis-Golgi-localized MTP11 (TC# 3.A.3.2.19) is non-functional (Farthing et al. 2023).  The trans-Golgi-localized MTP10 plays a role in Mn2+-toxicity tolerance, but only in mutants when MTP8 and MTP11 are non-functional. ().

Accession Number:Q9SY55
Protein Name:Calcium-transporting ATPase 3, endoplasmic reticulum-type
Length:998
Molecular Weight:109060.00
Species: [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Endosome membrane1 / Multi-pass membrane protein2
Substrate calcium(2+), manganese(2+)

Cross database links:

RefSeq: NP_563860.1   
Entrez Gene ID: 837550   
Pfam: PF00689    PF00690    PF00122    PF00702   
BioCyc: MetaCyc:MONOMER-14610   
KEGG: ath:AT1G10130   

Gene Ontology

GO:0012505 C:endomembrane system
GO:0005794 C:Golgi apparatus
GO:0016021 C:integral to membrane
GO:0031902 C:late endosome membrane
GO:0005524 F:ATP binding
GO:0005388 F:calcium-transporting ATPase activity
GO:0015410 F:manganese-transporting ATPase activity
GO:0046872 F:metal ion binding
GO:0006754 P:ATP biosynthetic process
GO:0006816 P:calcium ion transport
GO:0055071 P:manganese ion homeostasis
GO:0006828 P:manganese ion transport
GO:0048364 P:root development

References (4)

[1] “Two additional type IIA Ca(2+)-ATPases are expressed in Arabidopsis thaliana: evidence that type IIA sub-groups exist.”  Pittman J.K.et.al.   10433975
[2] “ECA3, a Golgi-localized P2A-type ATPase, plays a crucial role in manganese nutrition in Arabidopsis.”  Mills R.F.et.al.   18024560
[3] “A distinct endosomal Ca2+/Mn2+ pump affects root growth through the secretory process.”  Li X.et.al.   18567829
[4] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MEDAYARSVS EVLDFFGVDP TKGLSDSQVV HHSRLYGRNV LPEEKRTPFW KLVLKQFDDL 
61:	LVKILIVAAI VSFVLALANG ETGLTAFLEP FVILLILAAN AAVGVITETN AEKALEELRA 
121:	YQANIATVLR NGCFSILPAT ELVPGDIVEV TVGCKIPADL RMIEMSSNTF RVDQAILTGE 
181:	SCSVEKDVDC TLTTNAVYQD KKNILFSGTD VVAGRGRAVV IGVGSNTAMG SIHDSMLQTD 
241:	DEATPLKKKL DEFGSFLAKV IAGICVLVWV VNIGHFSDPS HGGFFKGAIH YFKIAVALAV 
301:	AAIPEGLPAV VTTCLALGTK KMARLNAIVR SLPSVETLGC TTVICSDKTG TLTTNMMSVS 
361:	KICVVQSAEH GPMINEFTVS GTTYAPEGTV FDSNGMQLDL PAQSPCLHHL AMCSSLCNDS 
421:	ILQYNPDKDS YEKIGESTEV ALRVLAEKVG LPGFDSMPSA LNMLSKHERA SYCNHYWENQ 
481:	FKKVYVLEFT RDRKMMSVLC SHKQMDVMFS KGAPESIIAR CNKILCNGDG SVVPLTAAGR 
541:	AELESRFYSF GDETLRCLAL AFKTVPHGQQ TISYDNENDL TFIGLVGMLD PPREEVRDAM 
601:	LACMTAGIRV IVVTGDNKST AESLCRKIGA FDNLVDFSGM SYTASEFERL PAVQQTLALR 
661:	RMTLFSRVEP SHKRMLVEAL QKQNEVVAMT GDGVNDAPAL KKADIGIAMG SGTAVAKSAS 
721:	DMVLADDNFA SIVAAVAEGR AIYNNTKQFI RYMISSNIGE VVCIFVAAVL GIPDTLAPVQ 
781:	LLWVNLVTDG LPATAIGFNK QDSDVMKAKP RKVGEAVVTG WLFFRYLVIG VYVGLATVAG 
841:	FIWWFVYSDG GPKLTYSELM NFETCALRET TYPCSIFEDR HPSTVAMTVL VVVEMFNALN 
901:	NLSENQSLLV ITPRSNLWLV GSIILTMLLH VLILYVHPLA VLFSVTPLSW AEWTAVLYLS 
961:	FPVIIIDELL KFLSRNTGMR FRFRLRKADL LPKDRRDK