4.C.1.1.19
Hybrid
PKS-NRPS synthetase; part of the gene cluster that mediates the
biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein
biosynthesis on ribosomes by suppressing the release of new protein
(Yun et al. 2015, Ninomiya et al. 2020, Yun et al. 2020). TAS1 alone is sufficient for TeA synthesis via the condensation of
isoleucine (Ile) with acetoacetyl-CoA by the N-termainal NRPS module and
subsequent cyclization conducted by the C-terminal KS domain (Yun et al. 2015, Yun et al. 2020).
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| Accession Number: | G4N137 |
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| Protein Name: | Hybrid PKS-NRPS synthetase TAS1 |
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| Length: | 1728 |
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| Molecular Weight: | 187803.00 |
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| Species: | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) [242507] |
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| Substrate |
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1: MYNRCCRKVW IQGRVAYRPA YIRQSPPQQT LYRGIDLLPF TTIVMDHQSG FQNPSPSGGL
61: MFSASAKRFI SRIVGFVGRG AEVQQKAVGL CPLKTERRRA AAGGLLLPTG LRMGRSIIMS
121: VRPLPFLTGP APSPDTAAGF KPSPPTGNLV SVSPLSKAQM ALWFDYLQHP TSTHYFLTLK
181: VELDKQPLSL DKIIQVIRGL GKQHAMLRTT FHVDTDTDDM SKSYMAVHDD SWDQEIHVLM
241: NDAQLYEALR KPFQLSSESP VRWVVQMKLQ PGSARSTYTV YAAGHHIGVD GASMSVLSNQ
301: LLEAVASEVE DQPDHSGPHY GDYIQRQARY LRSSAGAAAG RFWLSQLRHT QPFRWRMEPP
361: EEINTPNYRQ LDTWNFFPTA EIQEWGNLYK TSWFRVATSI VGLVTAAMAE PQAHHDHALM
421: VAFGARPRGF ENNVSHMANT MPVKFPLSSL LRDDATFSDA VKAMGRNVST AKKHENFPFM
481: SLMEQANRHM DPTLLDFKVA ITYSPKLANK SCELFPVEGI WDLFFCFLEQ EDGVALGVIS
541: NPRVFGAEAL GQLQSLFNEV FALSKARPSF KLSDLAFLQN RTPARFISGP ALDDVESISK
601: SRVYRLIKAR AASQPDLVAL MSAEKGVQMT YRELAAQSSQ VAHFLQKQRL CKGDAVLVHL
661: ERGFAQIVWI LGVMEAGACY VALDKTWPAA RKEAILRTAN GKLLVTDDEQ MDFEKQDTTV
721: VFLAPSAAEI ASMPQSTCEC EVADDDLAYV VFTSGSTGQP KGVMVEHSNL SHYVSATRSL
781: VKTGPHSRML QLASFAFDAI VLEYAVTLAH GGTLCFANHP EVLVGEYLAD VIDSNQVNFF
841: HCTPSVLSTL PAGRRLPSLR IVSVGGEASP PGLLDHWRKR VELLHAYGPT ECTVICTLES
901: LTQDESTQTA IDATVIGKAL PNLDIRICEE GKLEPLAPNQ VGEICVVGPQ VSRGYMGQEE
961: LTASKFHNIT LADGHPSRLY RTGDKGFIDD DGKLHIQGRI GNREIKVRGY RLDLYEVEKN
1021: VMAFDPEVTQ VSIQQVGESL VALVVPASID CDRIRSKLLK DMPRYAVPTR FIRVASLPLN
1081: TNGKIDHTQA SSLAAELVMH DTVLPTVDAT PTPTAAVRAV GVTEENLRLK TKENGMERQE
1141: MLRRHLTAEV TALWAKLLGS SRQFDPEVGF FDAGGHSLLL TQLHKLIKER FGTGSRPSLL
1201: DIFSMSSIRK QVDCLMGIVD QDAMLGSEPT GGSSSRSQSR RSAETSSSST SAPSSVPVDA
1261: ERNLYAIVGI SCRFPGANTA EQLWNVLMEQ RDAITTFCPA ENLGFALEEN SVFVPRYGMI
1321: DALKDFEPSA YSMSDAEAQT IDPQKRVFLD VAADALADAG TSASPGNPLD PVGVFVGAAT
1381: NTFLSSRDNP GSKPPGDEEP QSFANHYQQL LDCPIGTFAS FKLNLTGPVV TLNTACSSAL
1441: AALHLACASL SHGDCNAAVV GGVSMAYPQE GGYVTARPGG DSSAVFSPSG VCHPLDSRAD
1501: GCVPADGAAA LVIKRLADAR ADGCRVYAVI EGVAVSADGS DDKAGLGVPS SSGQSRTVEA
1561: ALRRAGPQAL SRLRYVEMHG SGTPWGDALE VQGLKMAFDR LSKTGAAEQS GTGRAQPEAD
1621: RIYLGSNKGN CGNTEAASGL LSLIKASMAL NLGVVPPLPN LAEPNPKCEF EETKFEPLGK
1681: QLALAPGDRV MNKRQRIVRS PNWISDATWF VISTVDNLLP KLTPAAVA