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8.A.120.2.1
The metastatic lymph node-64 (MLN-64; MLN64; STARD3; CAB1) protein mediates endosomal cholesterol transport to mitochondria and the plasma membrane (Kennedy et al. 2014). It has been implicated in toxin-induced resistance. Down-regulation of MLN64 in Niemann-Pick C1 deficient cells decreased mitochondrial cholesterol content, suggesting that MLN64 functions independently of NPC1 (Balboa et al. 2017), and overexpression increases the mitochondrial cholesterol content and decreases the mitochondrial glutathione content, leading to mitochondrial dysfunction (Balboa et al. 2017). STARD3 is a key protein in the cholesterol movement in cancer cells (Asif et al. 2021). STARD3 is also a lutein- and zeaxanthin (carotenoid)-binding protein (Arunkumar et al. 2020).

Accession Number:Q14849
Protein Name:MLN-64
Length:445
Molecular Weight:50502.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Late endosome membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

RefSeq: NP_006795.3   
Entrez Gene ID: 10948   
Pfam: PF10457    PF01852   
OMIM: 607048  gene

Gene Ontology

GO:0016021 C:integral to membrane
GO:0031902 C:late endosome membrane
GO:0015485 F:cholesterol binding
GO:0017127 F:cholesterol transporter activity
GO:0008203 P:cholesterol metabolic process
GO:0006869 P:lipid transport
GO:0006839 P:mitochondrial transport
GO:0006694 P:steroid biosynthetic process

References (5)

[1] “Identification of four novel human genes amplified and overexpressed in breast carcinoma and localized to the q11-q21.3 region of chromosome 17.”  Tomasetto C.L.et.al.   7490069
[2] “Isolation of a candidate gene, CAB1, for cholesterol transport to mitochondria from the c-ERBB-2 amplicon by a modified cDNA selection method.”  Akiyama N.et.al.   9270027
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Structure and lipid transport mechanism of a StAR-related domain.”  Tsujishita Y.et.al.   10802740
[5] “The steroidogenic acute regulatory protein homolog MLN64, a late endosomal cholesterol-binding protein.”  Alpy F.et.al.   11053434
Structure:
1EM2   2I92   5I9J     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MSKLPRELTR DLERSLPAVA SLGSSLSHSQ SLSSHLLPPP EKRRAISDVR RTFCLFVTFD 
61:	LLFISLLWII ELNTNTGIRK NLEQEIIQYN FKTSFFDIFV LAFFRFSGLL LGYAVLRLRH 
121:	WWVIAVTTLV SSAFLIVKVI LSELLSKGAF GYLLPIVSFV LAWLETWFLD FKVLPQEAEE 
181:	ERWYLAAQVA VARGPLLFSG ALSEGQFYSP PESFAGSDNE SDEEVAGKKS FSAQEREYIR 
241:	QGKEATAVVD QILAQEENWK FEKNNEYGDT VYTIEVPFHG KTFILKTFLP CPAELVYQEV 
301:	ILQPERMVLW NKTVTACQIL QRVEDNTLIS YDVSAGAAGG VVSPRDFVNV RRIERRRDRY 
361:	LSSGIATSHS AKPPTHKYVR GENGPGGFIV LKSASNPRVC TFVWILNTDL KGRLPRYLIH 
421:	QSLAATMFEF AFHLRQRISE LGARA