1.D.169. The Ion Transporting Carbon Nanodot (C-ND) Family

Carbon nanodots synthesized from beta-alanine (Ala-CDs) and detonation nanodiamonds (NDs) were examined (Shatursky et al. 2022). Ala-CDs and NDs increased the extracellular concentrations of neurotransmitters and decreased acidification of synaptic vesicles but did not change the lipid order of the membrane. Both nanoparticles, Ala-CDs and NDs, were capable of increasing the conductance of a black lipid membrane (BLM) by inducing stable potential-dependent cation-selective pores. Introduction of a divalent cation, Zn2+ or Cd2+ on the particles` application side (cis-side) increased the rate of Ala-CDs pore-formation in the BLM. The application of positive potential (+100 mV) to the cis-chamber with Ala-CDs or NDs also activated insertion as compared with a negative potential (-100 mV). The Ala-CD pores exhibited a wide-range distribution of conductances between 10 and 60 pS and a consecutive increase in conductance of each major peak by ~10pS, which suggested the clustering of the same basic ion-conductive structure. NDs also formed ion-conductive pores ranging from 6 pS to 60 pS with the major peak of conductance at ~12 pS in cholesterol-containing membranes. Observed Ala-CDs and NDs-induced increases in transmembrane current that coincided with the disturbance of excitatory and inhibitory neurotransmitter transport in nerve terminals (Shatursky et al. 2022).



Shatursky, O.Y., A.P. Demchenko, I. Panas, N. Krisanova, N. Pozdnyakova, and T. Borisova. (2022). The ability of carbon nanoparticles to increase transmembrane current of cations coincides with impaired synaptic neurotransmission. Biochim. Biophys. Acta. Biomembr 1864: 183817.