1.E.56 The Putative 3 TMS Holin (3-Hol) Family The 3-Hol family is large with thousands of members, all of small size and usually with 3 TMSs. Many family members are annotated as holins. They derive from proteobacteria and their phage.
References:
Roszniowski, B., A. Latka, B. Maciejewska, D. Vandenheuvel, T. Olszak, Y. Briers, G.S. Holt, M.A. Valvano, R. Lavigne, D.L. Smith, and Z. Drulis-Kawa. (2016). The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage. Appl. Microbiol. Biotechnol. [Epub: Ahead of Print]
Examples:
TC#	Name	Organismal Type	Example
1.E.56.1.1	Putative holin (DUF745 protein) of 102 aas and 3 TMSs.	Proteobacterial virus	*Holin of Salmonella* phage SPN1S**

1.E.56.1.10	Putative holin of 92 aas and 3 TMSs	Proteobacteria	Putative holin of Pseudomonas poae

1.E.56.1.11	Putative holin of 85 aas and 3 TMSs	Proteobacteria	Putative holin of Acinetobacter baumannii

1.E.56.1.12	Class I holin of 105 aas and 3 TMSs	Proteobacterial viru	*Class 1 hoin of Pseudomonas* phage AF**

1.E.56.1.2	Holin of 106 aas and 3 TMSs. Similar to the holins of phage KS5 and AP3 which have been used to combat infection by B. cenocepacia in a Galleria mellonella moth wax model. AP3 treatment of larvae infected with B. cenocepacia revealed an increase (P < 0.0001) in larval survival (Roszniowski et al. 2016).	Proteobacteria	Holin of Burkholderia cenocepacia

1.E.56.1.3	Putative holin of 93 aas and 3 TMSs	Proteobacterial virus	Holin of Enterobacterial phge mEp390

1.E.56.1.4	Uncharacterized protein of 93 aas and 3 TMSs	Proteobacteria	UP of Klebsiella variicola

1.E.56.1.5	Putative holin of 91 aas and 3 TMSs	Proteobacterial viru	Enterbacterial phage PhiP27

1.E.56.1.6	Putative holin of 93 aas and 3 TMSs	Proteobacteria	Putative holin of E. coli

1.E.56.1.7	Putative holin of 94 aas and 3 TMSs	Proteobacterial viru	*Putative holin of Pseudomonas* phage Phi297**

1.E.56.1.8	Putative holin of 94 aas and 3 TMSs	Proteobacteria	Putative holin of Laribacter hongkongensis

1.E.56.1.9	Putative holin, gp28, of 88 aas and 3 TMSs	Proteobacterial viru	*gp28 of Burkholderia* phage KS14**