8.A.236. The Sugarcane Mosaic Virus 6K2 Protein (ScMV-6K2) Family Sugarcane mosaic virus (SCMV), one of the main pathogens causing sugarcane mosaic disease, is widespread in sugarcane (Saccharum spp. hybrid) planting areas and causes heavy yield losses. RESPIRATORY BURST OXIDASE HOMOLOG (RBOH) NADPH oxidases and plasma membrane intrinsic proteins (PIPs) have been associated with the response to SCMV infection. Zhang et al. 2023 demonstrated that SCMV infection upregulates the expression of ScRBOHs and the accumulation of hydrogen peroxide (H₂O₂), which inhibits SCMV replication. All eight sugarcane PIPs (ScPIPs) interacted with SCMV-encoded protein 6K2, whereby two PIP2s (ScPIP2;1 and ScPIP2;4) were verified as capable of H₂O₂ transport. SCMV-6K2 interacts with ScPIP2;4 via TMS 5 to interfere with the oligomerization of ScPIP2;4, subsequently impairing ScPIP2;4 transport of H2O2.This is the mechanism adopted by SCMV to employ 6K2 to counteract the host resistance mediated by H₂O₂ to facilitate virus infection, and it provides potential molecular targets for engineering sugarcane resistance against SCMV.
References:
Zhang, H., Z. Yang, G. Cheng, T. Luo, K. Zeng, W. Jiao, Y. Zhou, G. Huang, J. Zhang, and J. Xu. (2023). Sugarcane mosaic virus employs 6K2 protein to impair ScPIP2; 4 transport of H₂O2 to facilitate virus infection. Plant Physiol. [Epub: Ahead of Print]
Examples:
TC#	Name	Organismal Type	Example
8.A.236.1.1	Sugar cane mosaic virus 6K2 protein of 53 aas and 1 C-terminal TMS. Sugarcane mosaic virus (SCMV), one of the main pathogens causing sugarcane mosaic disease, is widespread in sugarcane (Saccharum spp. hybrid) planting areas and causes heavy yield losses. RESPIRATORY BURST OXIDASE HOMOLOG (RBOH) NADPH oxidases and plasma membrane intrinsic proteins (PIPs) have been associated with the response to SCMV infection. Zhang et al. 2023 demonstrated that SCMV infection upregulates the expression of ScRBOHs and the accumulation of hydrogen peroxide (H₂O₂), which inhibits SCMV replication. All eight sugarcane PIPs (ScPIPs) interacted with SCMV-encoded protein 6K2, whereby two PIP2s (ScPIP2;1 and ScPIP2;4) were verified as capable of H₂O₂ transport. SCMV-6K2 interacts with ScPIP2;4 via TMS 5 to interfere with the oligomerization of ScPIP2;4, subsequently impairing ScPIP2;4 transport of H2O2.This is the mechanism adopted by SCMV to employ 6K2 to counteract the host resistance mediated by H₂O₂ to facilitate virus infection, and it provides potential molecular targets for engineering sugarcane resistance against SCMV.		The 6K2 protein of sugarcane mosaic virus

8.A.236.1.2	6K2 protein of 53 aas with 1 C-terminal TMS.		6K2 protein of Chilli ringspot virus

8.A.236.1.3	6K2 protein of 53 aas and 1 TMS.		6K2 protein of Donkey orchid virus A

8.A.236.1.4	The 6K2 proteiin of 53 aas and 1 TMSs.		6K2 protein of Canna yellow streak virus