9.A.22 The Putative Bacillus Transporter of Unknown Function (BTUF) Family

A single 6 TMS protein, originally termed NhaE of Bacillus pseudofirmus OF4, had been reported in TCDB as an exchanger of H+ for either Na+ or K+ , but the results were not reproduced and a paper was not published. Members of this family are therefore of unknown function.



This family belongs to the MusI (MusI) Superfamily.

 

References:

Cheng, J., A.A. Guffanti, and T.A. Krulwich. (1997). A two-gene ABC-type transport system that extrudes Na+ in Bacillus subtilis is induced by ethanol or protonophore. Mol. Microbiol. 23: 1107-1120.

Examples:

TC#NameOrganismal TypeExample
9.A.22.1.1

The NhaE Na+/K+:H+ antiporter.  The gene encoding this protein is adjacent to genes encoding a GntR transcriptional regulator, two ABC ATPases designated NatA1 and NatA2 and an ABC membrane protein designated NatB, all possibly within a single operon.

Bacteria

NhaE (orf 14) of Bacillus pseudofirmus

 
9.A.22.1.2

Hypothetical protein.  The encoding gene is adjacent to genes encoding a GntR transcriptional regulator (Q9KDP7), two ABC ATPases (Q9KDP6 and 7) and a putative ABC membrane protein (Q9KDP5), all possibly in a single operon.

Firmicutes

HP of Bacillus halodurans

 
9.A.22.1.3

Hypothetical protein.  Encoded by a gene adjacent to genes encoding a GntR transcriptional regulator, a similar membrane protein with 6 TMSs in a 2 + 4 arrangement, followed by two genes encoding two ABC ATPases annotated as NatA1 and A2, followed by a membrane protein annotated as NatB2.  These genes may all be in a single operon.

Firmicutes

HP of Bacillus cellulosilyticus

 
9.A.22.1.4

Uncharacterized protein of 241 aas and 6 TMSs.

UP of Alkalibacillus haloalkaliphilus

 
9.A.22.1.5

Uncharacterized protein of 251 aas and 6 TMSs in a 2 + 4 TMS arrangement.

UP of Salilacibacter sp. NKC3-5

 
9.A.22.1.6

Uncharacterized protein of 243 aas and 6 TMSs in a 2 + 2 + 2 TMS arrangement.

UP of Geomicrobium sp. JCM 19039

 
9.A.22.1.8

Putative 3-component ABC transporter with two membrane proteins, both of 6 TMSs in a 2 + 4 TMS arrangement and a single ATPase. This system is encoded by 3 genes that are adjacent to each other and a GntR transcription factor gene, and also to a putative Na+ ABC efflux system, NatAB (see 3.A.1.115.4; Cheng et al. 1997).

Putative 3 component ABC transport system with two membrane proteins (E6TQK3 and 4), and one ATPase (E6TQK6), possibly regulated at the GntR-type transcriptional factor (E6TQK5) of Evansella cellulosilytica (Bacillus cellulosilyticus)

 
Examples:

TC#NameOrganismal TypeExample
9.A.22.2.1

Uncharacterized protein of 243 aas and 6 TMSs.

Firmicutes

UP of Bacillus clausii

 
9.A.22.2.2

Uncharacterized protein of 240 aas ans 6 TMSs

UP of Alkalihalobacillus lonarensis

 
Examples:

TC#NameOrganismal TypeExample
9.A.22.3.1

Uncharacterized 6 TMS protein.  Clearly a member of the 9.A.22 family, but also exhibits sequence similarity to the second half (TMSs 7 - 12) of the MFS permease, 2.A.1.62.3.

Firmicutes

UP of Paenibacillus terrae

 
9.A.22.3.2

Uncharacterized protein of 235 aas and 6 TMSs.

UP of Thermoactinomyces sp. DSM 45892

 
9.A.22.3.3

Uncharacterized protein of 249 aas and 6 TMSs.

UP of Salinibacillus kushneri

 
9.A.22.3.4

Uncharacterized protein of 229 aas and 6 TMSs.

UP of Thermomicrobiales bacterium (activated sludge metagenome)

 
9.A.22.3.5

Uncharacterized protein of 241 aas and 6 TMSs

UP of Paenibacillus montaniterrae

 
9.A.22.3.6

Uncharacterized protein of 248 aas and 6 TMSs in a 2 + 3 + 1 TMS arrangement.

UP of Hazenella coriacea

 
Examples:

TC#NameOrganismal TypeExample
9.A.22.4.1

Uncharacterized protein of 237 aas and 6 TMSs.

UP of Chryseobacterium gleum

 
9.A.22.4.2

Uncharacterized protein of 224 aas and 6 TMSs

UP of Sphingopyxis sp. MC1

 
9.A.22.4.3

Uncharacterized protein of 229 aas and 6 TMSs

UP of Ensifer adhaerens (Sinorhizobium morelense)

 

 
Examples:

TC#NameOrganismal TypeExample
9.A.22.5.1

Uncharacterized protein of 300 aas and 6 TMSs in a 2 + 2 + 2 TMS arrangement.

UP of Candidatus Peribacter riflensis (groundwater metagenome)

 
9.A.22.5.2

Uncharacterized protein of 358 aas and 6 TMSs in a 2 + 2 + 2 TMS arrangement.

UP of Candidatus Gracilibacteria bacterium CG2_30_37_12 (groundwater metagenome)

 
9.A.22.5.3

Uncharacterized protein of 318 aas and 7 or 6 TMSs in a 2 + 2 + 2 or 3 TMS arrangement.

UP of Candidatus Magasanikbacteria bacterium (groundwater metagenome)

 
9.A.22.5.4

Uncharacterized protein of 343 aas and 7 TMSs in a 2 + 2 + 3 TMS arrangement.

UP of Anaerolineales bacterium (bioreactor metagenome)

 
9.A.22.5.5

Uncharacterized protein of 349 aas and 7 TMSs in a 1 + 2 + 2 + 2 TMS arrangement.

UP of Microgenomates group bacterium

 
9.A.22.5.6

Uncharacterized protein of 375 aas and 6 TMSs in a 2 + 2 + 2 TMS arrangement.

UP of Coriobacteriia bacterium (volcano metagenome)

 
9.A.22.5.7

Uncharacterized protein of 403 aas and 6 TMSs

UP of Hazenella coriacea

 
9.A.22.5.8

Uncharacterized protein of 452 aas and 6 or 7 TMSs in a 2 + 2 + 2 or 3 TMS arrangement.

UP of Haloprofundus marisrubri

 
9.A.22.5.9

Uncharacterized protein of 397 aas and 6 or 7 TMSs in a 2 + 2 + 2 or 3 TMS arrangement.

UP of Leptospiraceae bacterium (activated sludge metagenome)