9.B.128 The O-antigen Polymerase, WzyE (WzyE) Family

The WzyX translocase (2.A.66.2.12) (an isoprenoid lipid sugar glucan flippase) forms a complex with Wzy (WzyE) and Wzz for assembly of periplasmic O-antigen in lipopolysaccharide (Marolda et al., 2006). WzyE (450aas; 11-13 TMSs) is referred to as the enterobacterial common antigen (ECA) polysaccharide chain elongation polymerase (Kajimura et al., 2005). However, it and its homologues 'look like' a permease and show appreciable sequence similarity with MFS and MATE carriers following TC BLAST searches. This family may be distantly related to 9.B.67, also designated as an O-antigen polymerase family.


This family belongs to the O-Antigen Polymerase (OAPol) Superfamily.
 

References:

Kajimura, J., A. Rahman, and P.D. Rick. (2005). Assembly of cyclic enterobacterial common antigen in Escherichia coli K-12. J. Bacteriol. 187: 6917-6927.
Luo, F., X. Sun, Z. Qu, and X. Zhang. (2016). Salmonella typhimurium-induced M1 macrophage polarization is dependent on the bacterial O antigen. World J Microbiol Biotechnol 32: 22.
Marolda, C.L., L.D. Tatar, C. Alaimo, M. Aebi, and M.A. Valvano. (2006). Interplay of the Wzx translocase and the corresponding polymerase and chain length regulator proteins in the translocation and periplasmic assembly of lipopolysaccharide o antigen. J. Bacteriol. 188: 5124-5135.
Examples:

TC#NameOrganismal TypeExample
9.B.128.1.1

WzyE O-antigen polymerase (Marolda et al., 2006).

Bacteria

WzyE of E. coli (B614D1)

 
Examples:

TC#NameOrganismal TypeExample
9.B.128.2.1

Hypothetical protein Aazo_4920 (481aas; 12 putative TMSs)

Bacteria

Aazo_4920 of Nostoc azollae (D7DZ35)

 
9.B.128.2.2

Uncharacterized protein of 506 aas and 12 TMSs.

UP of Sphingobacteriales bacterium 44-61 (bioreactor metagenome)

 
Examples:

TC#NameOrganismal TypeExample
9.B.128.3.1

O-antigen polymerase, Rfc. May link the O-antigen tetrasaccharide units into long chains, giving rise to typical smooth LPS This LPS is essential for M1 macrophage polarization (Luo et al. 2016).

Bacteria

O-antigen polymerase of Salmonella typhi (P0A236)

 
Examples:

TC#NameOrganismal TypeExample