9.B.287.  The Fat Storage-inducing Transmembrane Protein 2 (FIT2) Family 

FIT2 aids in partitioning of cellular triacylglycerol into lipid droplets. A genome-wide association study reported the FITM2-R3H domain containing like-HNF4A locus to be associated with type 2 diabetes (T2DM) in East Asian populations. Mice with adipose tissue (AT)-specific FIT2 knockout exhibited lipodystrophic features, with reduced AT mass, insulin resistance, and greater inflammation in AT when fed a high-fat diet. Agrawal et al. 2018 found FIT2 protein abundance is lower in subcutaneous and omental AT obtained from patients with T2DM compared with nondiabetic control subjects. Partial loss of FIT2 protein in primary human adipocytes attenuated their lipid storage capacity and induced insulin resistance. After palmitate treatment, triacylglycerol accumulation, insulin-induced Akt (Ser-473) phosphorylation, and insulin-stimulated glucose uptake were significantly reduced in FIT2 knockdown adipocytes compared with control cells. FIT2 is associated with T2DM in humans and plays an integral role in maintaining metabolically healthy AT function (Agrawal et al. 2018).



Agrawal, M., C.R. Yeo, A. Shabbir, V. Chhay, D.L. Silver, F. Magkos, A. Vidal-Puig, and S.A. Toh. (2018). Fat storage-inducing transmembrane protein 2 (FIT2) is less abundant in type 2 diabetes, and regulates triglyceride accumulation and insulin sensitivity in adipocytes. FASEB J. fj201701321RR. [Epub: Ahead of Print]

Cen, O. and R. Longnecker. (2015). Latent Membrane Protein 2 (LMP2). Curr Top Microbiol Immunol 391: 151-180.

Naser, I., Y. Yabu, Y. Maeda, and T. Tanaka. (2022). Highly Efficient Genetic Transformation Methods for the Marine Oleaginous Diatom Fistulifera solaris. Mar Biotechnol (NY). [Epub: Ahead of Print]


TC#NameOrganismal TypeExample

The Fit2 protein of 262 aas and 5 TMSs in a 3 + 2 arrangement.  Functions in fat storage and lipid droplet formation (Agrawal et al. 2018).

Fit2 of Homo sapiens


Uncharacterized FIT2 homologue of 362 aas and 6 TMSs in a 1 + 2 + 1 + 2 arrangement.

UP of Myceliophthora thermophila (Sporotrichum thermophile)


Uncharacterized protein of 352 aas and 7 TMSs in a 3 + 1 + 1 + 2 TMS arrangement.

UP of Tetranychus urticae (Two-spotted spider mite)


Uncharacterized protein of 274 aas and 6 TMSs in a 2 + 1 + 3 TMS arrangement, Yft2.

Yft2 of Saccharomyces cerevisiae (Baker's yeast)


Fat storage-inducing transmembrane protein 1, FIT1, of 292 aas and 6 TMSs in a 3 + 3 TMS arrangement (Naser et al. 2022).

FIT1 of Mus musculus


TC#NameOrganismal TypeExample