9.B.29 The 4-5 TMS Putative Chaperone (4-5PC) Family
The 4-5PC family consists of proteins from Spirochaetes (Leptospira (subfamily 1)) and many potentially distant homologues in other bacteria (subfamilies 2 - 4) as well as archaea (subfamily 5). They have 200 - 270 aas with 4 - 5 TMSs. Some of them are encoded adjacent to genes coding for membrane fusion proteins (MFPs; TC# 8.A.1) and outer menbrane factors (OMFs; TC# 1.B.17) as well as ABC transporters (Yamada et al. 2012). They may be important for assembly of multicomponent transporters such as ABC systems (Yamada et al. 2012). These proteins may be distantly related to members of the ABC superfamily (TC# 3.A.1), as well as members of the golgi Yip1 family (TC# 9.B.135) and the UAB5 family (TC# 9.B.333), but these suggestions have not been confirmed.
References:
Uncharacterized protein of 205 aas and 5 TMSs
Spirochaetes
UP of Leptospira interogans
Uncharacterized protein of 204 aas and 5 TMSs
Spirochaetes
UP of Leptospira biflexa
Uncharacterized protein of 202 aas and 5 TMSs.
UP of Leptospiraceae bacterium (activated sludge metagenome)
Uncharacterized protein of 231 aas and 5 TMSs.
Firmicutes
UP of Bacillus sp.
Uncharacterized protein of 227 aas and 5 TMSs
Spirochaetes
UP of Leptospira santarosai
Uncharacterized protein of 198 aas and 5 TMSs
Euryarchaea
UP of Methanocorpusculum labreanum
Uncharacterized putative ABC-type transporter with three constituents, one of 194 aas and 5 TMSs (A3U434), one encoding another hydrophobic protein of 161 aas and 4 TMSs (A3U435) and another gene encoding an ABC-type ATPase of 251 aas (SufC; A3U437) in the same gene cluster.
Proteobacteria
Putative ABC efflux transporter of Pseudooceanicola batsensis (Oceanicola batsensis)
Yos1 protein of the Pfam Yip1 superfamily of 220 aas and 5 TMSs. Yos1 is a subunit of the Yip1-Yif1 complex. Its loss blocks transfer between the ER and the Golge apparatus (Heidtman et al. 2005).
Firmicutes
UP of Caldicellulosiruptor saccharolyticus
Uncharacterized protein of 196 aas and 5 TMSs.
Actinobacteria
UP of Rubrobacter xylanophilus
Uncharacterized protein of 221 aas and 5 TMSs. Also similar to 3.A.1.122.2.
Firmicutes
UP of Carboxydothermus hydrogenoformans
Uncharacterized protein of 267 aas and 5 TMSs with an N-terminal zinc_ribbon_2 domain.
UP of Candidatus Lokiarchaeota archaeon CR_4
YknW, a membrane protein of 231 aas and 5 TMSs, is part of an unusual four-component ABC transporter,
which is required for protection against the killing factor SdpC
(sporulation-delaying protein). YknW plays an essential role in the assembly of the YknXYZ ABC transport complex (TC# 3.A.1.122.2) and interacts directly with it (Yamada et al. 2012).
YknW of Bacillus subtilis
YIP family protein of 245 aas and 5 TMSs.
YIP of Gemmatirosa kalamazoonesis
Uncharacterized protein of 199 aas and 5 TMSs.
UP of Staphylococcus agnetis
Uncharacterized protein of 203 aas and 5 TMSs
Firmicutes
UP of Solibacillus silvestris (Bacillus silvestris)
YIP family protein of 316 aas and 5 TMSs.
YIP of Terriglobus roseus
Uncharacterized protein of 229 aas and 5 TMSs. This protein show weak similarity with a membeer of family 9.B.135 (the eukaryotic Yip family).
UP of Arenimonas caeni
Uncharacterized protein of 250 aas and 5 TMSs
UP of Thermoprotei archaeon
Uncharacterized protein of 216 aas and 5 TMSs
Firmicutes
UP of Bacillus cereus
Uncharacterized protein of 198 aas and 5 TMSs
Firmicutes
UP of Heliobacterium modesticaldum
Uncharacterized protein of 207 aas and 5 TMSs
Firmicutes
UP of Ammonifex degensii
Uncharacterized protein of 251 aas and 5 TMSs
Firmicutes
UP of Desulfotomaculum carboxydivorans
Uncharacterized protein of 212 aas and 5 TMSs (B8GHI5). The adjacent gene encodes a homologue of 209 aas and 5 TMSs (B8GHI4; e-41), then a 415 aas protein with 2 TMSs, N- and C-terminal (B8GHI3), then an ABC ATPase of 234 aas (B8GHI2), then a type 3 membrane ABC protein of 4 TMSs (B8GHI1) and then an inner membrane PrsW type protease (B8GHI0). B8GHI2 and B8GHI1 appear to comprise an ABC transporter assigned TC# 3.A.1.122.21. This protein may be a chitinase (see also TC# 1.E. (Krone et al. 2023).
Euryarchaea
UP of Methanosphaerula palustris
Uncharacterized protein of 210 aas and 4 TMSs
Euryarchaea
UP of Methanosarcina barkeri
Uncharacterized protein of 174 aas and 4 TMSs
Euryarchaera
UP of Archaeoglobus fulgidus
Uncharacterized protein of 203 aas and 5 TMSs
Proteobacteria
UP of Pseudomonas fluorescens
Uncharacterized protein of 203 aas and 5 TMSs with UniProt acc# of F9ZP17. F9ZP16 is a Membrane Fusion Protein (MFP: TC#8.A.1) and F9ZP15 is an outer membrane factor (OMF; TC# 1.B.17). There is no direct evidence that these three proteins function together.
Proteobacteria
UP of Acidithiobacillus caldus
Uncharacterized protein of 199 aas and 5 TMSs
Proteobacteria
UP of Aromatoleum aromaticum
Uncharacterized protein of 386 aas and 5 TMSs
Proteobacteria
UP of Sphingobium sp. SYK-6
Uncharacterized protein of 202 aas and 5 TMSs
Proteobacteria
UP of Alkalilimnicola ehrlichii
Uncharacterized protein of 181 aas and 5 TMSs
Bacteroidetes
UP of Bacteroides ovatus
Uncharacterized protein of 191 aas and 5 TMSs
Deinococcus/Thermus
UP of Deinococcus maricopensis
Uncharacterized protein of 175 aas and 5 TMSs
UP of Deinococcus cellulosilyticus
Yip family protein of 336 aas and 5 C-terminal TMSs.
Yip family protein of Dictyobacter aurantiacus
Uncharacterized protein of 226 aas and 5 TMSs
UP of Candidatus Altiarchaeales archaeon
DUF1282 domain-containing protein of 226 aas and 5 TM
DUF1282 protein of Litorilinea aerophila
Uncharacterized protein of 168 aas and 4 TMSs in a 2 + 2 TMS arrangement
UP of Hydrocarboniphaga effusa