9.B.98 The DUF95 (DUF95) Family

The DUF95 family consists of proteins that exhibit 4, 5 or 6 TMSs. They usually contain about 200 aas but can have N-terminal hydrophilic extensions of >100 aas. Members are found in archaea and bacteria. DUF95 domains are found in a few ABC transport systems such as 3.A.1.156.1 where the system may have an extra 4 TMS subunit.  Another ABC transport system having this domain is the 18 TMS protein (3.A.1.144.4) which has a 6 + 6 + 6 arrangement, and the last 6 TMSs correspond to the DUF95 domain.   It has been suggested that DUF95 proteins are auxilary subunits of bacteriocin ABC exporters (van Belkum et al., 2011). These proteins may be distantly related to TatC (2.A.64).


 

References:

Chastanet, A. and R. Losick. (2007). Engulfment during sporulation in Bacillus subtilis is governed by a multi-protein complex containing tandemly acting autolysins. Mol. Microbiol. 64: 139-152.

Gutierrez, J., R. Smith, and K. Pogliano. (2010). SpoIID-mediated peptidoglycan degradation is required throughout engulfment during Bacillus subtilis sporulation. J. Bacteriol. 192: 3174-3186.

Kemperman, R., M. Jonker, A. Nauta, O.P. Kuipers, and J. Kok. (2003). Functional analysis of the gene cluster involved in production of the bacteriocin circularin A by Clostridium beijerinckii ATCC 25752. Appl. Environ. Microbiol. 69: 5839-5848.

Labay, V., R.M. Weichert, T. Makishima, and A.J. Griffith. (2010). Topology of transmembrane channel-like gene 1 protein. Biochemistry 49: 8592-8598.

van Belkum, M.J., L.A. Martin-Visscher, and J.C. Vederas. (2011). Structure and genetics of circular bacteriocins. Trends Microbiol. 19: 411-418.

Examples:

TC#NameOrganismal TypeExample
9.B.98.1.1

PF1633 protein (185aas; 5 putative TMSs)

Archaea

PF1633 of Pyrococcus furiosus (Q8U0F6)

 
9.B.98.1.2

CPR_0765 (183aas; 5 TMSs)

Bacteria

CPR_0765 of Clostridium perfringens (Q0SUW6)

 
9.B.98.1.3

DUF95 family protein (191aas; 5 TMSs)

Bacteria

DUF95 protein of Frankia sp. (Ccl3) (Q2JF02)

 
9.B.98.1.4

Uncharacterized protein of 165 aas and 4 TMSs.

Euryarchaea

UP of Pyrococcus horikoshii

 
9.B.98.1.5

Uncharacterized protein of 214 aas and 4 or 5 TMSs

Euryarchaea

UP of Methanocaldococcus jannaschii

 
9.B.98.1.6

Uncharacterized protein of 168 aas and 4 TMSs.

Firmicutes

UP of Brochothrix campestris

 
9.B.98.1.7

Uncharacterized protein of 367 aas and 7 putative TMSs.

Euryarchaea

UP of Halobiforma lacisalsi

 
9.B.98.1.8

Uncharacterized protein of 207 aas and 4 TMSs.

UP of Lactobacillus rhamnosus

 
9.B.98.1.9

Stage II sporulation protein M of 335 aas and 6 TM

Sporulation of Alcanivorax gelatiniphagus

 
Examples:

TC#NameOrganismal TypeExample
9.B.98.2.1

Stage II Sporulation Protein M (SpoIIM) (Forms a complex with SpoIID and SpoIIP, both autolysins that help reshape septal peptidoglycan (Gutierrez et al., 2010; Chastanet and Losick, 2007)

Bacteria

SpoIIM of Bacillus subtilis (P37873)

 
Examples:

TC#NameOrganismal TypeExample
Examples:

TC#NameOrganismal TypeExample