3.A.9.1.4
The 20 component Ycf2-FtsHi chloroplast protein import motor complex:

The Ycf2-FtsHi complex functions as a TIC complex-associated ATPase motor in chloroplast protein import. Cryo-EM structure of the native Chlamydomonas reinhardtii Ycf2-FtsHi complex revealed up to 19 or 20 subunits, including conserved heterohexameric motor components and multiple green-algae-specific components (). This offers evolutionary insights into the conservation and diversity of the chloroplast import motor across species. 

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  Structure of the Chlamydomonas Ycf2-FtsHi complex revealed its composition and assembly.
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  ATP binding induces a conformational changes in the ATPase domain of Ctap1.
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  The preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro.
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  Ycf2-FtsHi exhibits evolutionary diversity between green algae and land plants,