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4.A.6.1.17
D-glucosaminate group translocating uptake porter, DgaABCD (IIA-141 aas, IIB-161 aas, IIC-249 aas, and IID-285 aas, respectively) (Miller et al. 2013).  Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) uses d-glucosaminate (2-amino-2-deoxy-d-gluconic acid) as a carbon and nitrogen source via DgaABCD (d-glucosaminate PTS permease components EIIA, EIIB, EIIC, and EIID). Two other genes in the dga operon (dgaE and dgaF) are required for wild-type growth with d-glucosaminate. Transcription of dgaABCDEF is dependent on RpoN (σ54) and an RpoN-dependent activator gene, dgaR. Introduction of a plasmid bearing dgaABCDEF under the control of the lac promoter into E. coli strains allowed them to grow on minimal medium containing d-glucosaminate. d-Glucosaminate is transported and phosphorylated at the C-6 position by DgaABCD. DgaE converts the resulting d-glucosaminate-6-phosphate to 2-keto-3-deoxygluconate 6-phosphate (KDGP), which is subsequently cleaved by the aldolase DgaF to form glyceraldehyde-3-phosphate and pyruvate. DgaF catalyzes the same reaction as that catalyzed by Eda, a KDGP aldolase in the Entner-Doudoroff pathway, and the two enzymes can substitute for each other in their respective pathways. Orthologs of the dga genes are largely restricted to certain enteric bacteria and a few Firmicutes (Miller et al. 2013).

Accession Number:D0ZLR5
Protein Name:Putative phosphotransferase system enzyme IIC
Length:249
Molecular Weight:26785.00
Species:Salmonella typhimurium (strain 14028s / SGSC 2262) [588858]
Number of TMSs:6
Substrate D-glucosaminate

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FASTA formatted sequence
1:	MDTLLFASLM GLYYWFARLR LGYTFSAMLL QPVVIAVFVG LLLGNMQTAM IIGAGMQLVY 
61:	LGVTSTPGGN VPSDPALAAC ISIPIAVKAG MDPNLAIALA IPFGVIGVFL DQLRRTLNAA 
121:	WVHMADKHAE TANMAGIMRC AFLYPALLGL VLRFPVVFAA NYFGQDVVES FLKLMPHWLT 
181:	HSFEIMGGIL PALGFAITIM VIGKKSLLPW FIGGFFAVLY LKVDIMAMAI FGTCVAFLIK 
241:	GLAKNEGAA