2.A.6.2.5
Fatty acid, bile salt, gonadal steroid, antibacterial peptide efflux pump, MtrCDE (Kamal et al., 2007). Opening of the outer membrane protein channel, MtrE, in the tripartite efflux pump, MtrCDE, is induced by interaction with the membrane fusion partner, MtrC (Janganan et al., 2011). The crystal structure of the trimeric MtrE forms a
vertical tunnel extending down contiguously from the outer membrane surface to the periplasmic end in the open conformational state of this channel (Lei et al. 2014). Coordination of substrate binding and protonation in MtrD controls the functionally rotating transport mechanism (Fairweather et al. 2021). The amino acyl sequence, N917-P927, plays a key role in modulating substrate access to the binding cleft and influences the overall orientation of the protein within the inner membrane necessary for optimal functioning (Chitsaz et al. 2021).
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| Accession Number: | Q51073 |
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| Protein Name: | MtrD |
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| Length: | 1067 |
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| Molecular Weight: | 113905.00 |
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| Species: | Neisseria gonorrhoeae [485] |
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| Number of TMSs: | 12 |
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| Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 |
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| Substrate |
fatty acid, bile acid |
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| Pfam: |
PF00873
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[1] “The MtrD protein of Neisseria gonorrhoeae is a member of the resistance/nodulation/division protein family constituting part of an efflux system.” Hagman K.E. et.al. 9245801
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1: MAKFFIDRPI FAWVISIFII AAGIFGIKSL PVSQYPSVAA PTITLHAIYP GASAQVMEGS
61: VLSVIERNMN GVEGLDYMST SADSSGSGSV SLTFTPDTDE NLAQVEVQNK LSEVLRTLPA
121: TVQQYGVTVS KARSNFLMIV MLSSDVQSTE EMNDYAQRNV VPELQRIEGV GQVRLFGAQR
181: AMRIWVDPKK LQNYNLSFAD VGSALSAQNI QISAGSIGSL PAVRGQTVTA TVTAQGQLGT
241: AEEFGNVILR ANTDGSNIYL KDVAKVGLGM EDYSSSTRLN GVNTTGMAVM LSNSGNAMAT
301: AKAVKERLAV LEKYFPQGMS WKTPYDTSKF VEISIEKVIH TLIEAMVLVF VVMYLFLQNI
361: RYTLIPTIVV PISLLGGFAF ISYMGMSINV LTMFAMILVI GIVVDDAIVV VENVERIMAG
421: EGLPPKEATK KAMGQISGAV IGITAVLISV FVPLAMFSGA AGNIYKQFAL TMASSIAFSA
481: FLALTLTPAL CATMLKTIPK GHHEEKKGFF GWFNKKFDSW THGYEGRVAK VLRKTFRMMV
541: VYIGLAVVGV FLFMRLPTSF LPTEDQGFVM VSVQLPAGAT KERTDATLAQ VTQLAKSIPE
601: IENIITVSGF SFSGSGQNMA MGFAIFKDWN ERTASGSDAV AVAGKLTGMM MGTLKDGFGI
661: AVVPPPILEL GNGSGLSINL QDRNNTGHTG IAGEGNELIQ KMRASGLFDP STVRAGGLED
721: SPQLKIDINR AAAAAQGISF ADIRTALASA LSSSYVSDFP NQGRLQRVMV QADEDARMQP
781: ADILNLTVPN KSGVAVPLST IATVSWENGT EQSVRFNGYP SMKLSASPAT GVSTGQAMEA
841: VQKMVDELGG GYSFEWGGQS SEEAKGGSQT LILYGLAVAA VFLVLAALYE SWSIPLAVIL
901: VIPLGLIGAA AGVTGRNLFE GLLGSVPSFA NDIYFQVGFV TVMGLSAKNA ILIIEFAKDL
961: QAQGKSAVEA ALEAARLRFR PIIMTSFAFI LGVVPLYIAA GASSASQRAI GTTVFWGMLV
1021: GTLLSVFLVP LFYVVVRKFF KETAHEHEMA VRHASKAGIT GSDDKQY