TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.1.19.1
Alkalinizatioin-activated Ca2+-selective channel, sperm-associated cation channel, CatSper, required for male fertility and the hyperactivated motility of spermatozoa (Kirichok et al. 2006). These channels require auxiliary subunits, CatSperβ, γ and δ for activity (Chung et al., 2011).  The primary channel protein is CatSper1 (Liu et al., 2007), and it may be a target for immunocontraception (Li et al. 2009). CatSper channels have been reported to regulate sperm motility (Vicente-Carrillo et al. 2017). Sperm competition is selective for a disulfide-crosslinked macromolecular architecture. CatSper channel opening occurs in response to pH, 2-arachidonoylglycerol, and mechanical force. A flippase function is hypothesized, and a source of the concomitant disulfide isomerase activity is found in CatSper-associated proteins beta, delta and epsilon (Bystroff 2018). More recently, it has been reported that rotational motion and rheotaxis of human sperm do not require functional CatSper channels or transmembrane Ca2+ signaling (Schiffer et al. 2020). Instead, passive biomechanical and hydrodynamic processes may enable sperm rolling and rheotaxis, rather than calcium signaling mediated by CatSper or other mechanisms controlling transmembrane Ca2+ flux. The Ca2+ channel CatSper is not activated by cAMP/PKA signaling but directly affected by chemicals used to probe the action of cAMP and PKA (Wang et al. 2020). The cation channel of sperm (CatSper) is essential for sperm motility and fertility. CatSper comprises the pore-forming proteins CATSPER1-4 and multiple auxiliary subunits, including CATSPERbeta, gamma, delta, epsilon, zeta, and EFCAB9. Lin et al. 2021 reported the cryo-EM structure of the CatSper complex isolated from mouse sperm. CATSPER1-4 conform to the conventional domain-swapped voltage-gated ion channel fold, following a counterclockwise arrangement. The auxiliary subunits CATSPERbeta, gamma, delta and epsilon - each of which contains a single transmembrane segment and a large extracellular domain - constitute a pavilion-like structure that stabilizes the entire complex through interactions with CATSPER4, 1, 3 and 2, respectively. The EM map revealed several previously uncharacterized components, exemplified by the organic anion transporter SLCO6C1. Lin et al. 2021 named this channel-transporter ultracomplex the CatSpermasome. The assembly and organizational details of the CatSpermasome lay the foundation for the development of CatSpermasome-related treatments for male infertility and non-hormonal contraceptives. CatSper is a target for inhibition, for use in male contraception, causing inhibition of sperm motility (Mariani et al. 2023). A CUG-initiated CATSPERθ functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking (Huang et al. 2023).

Accession Number:Q8NEC5
Protein Name:Cation channel sperm-associated protein 1
Length:780
Molecular Weight:90091.00
Species: [9606]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cell projection1 / Multi-pass membrane protein2
Substrate calcium(2+)

Cross database links:

RefSeq: NP_444282.3   
Entrez Gene ID: 117144   
Pfam: PF00520   
OMIM: 606389  gene
KEGG: hsa:117144   

Gene Ontology

GO:0005929 C:cilium
GO:0020017 C:flagellar membrane
GO:0016021 C:integral to membrane
GO:0005515 F:protein binding
GO:0006816 P:calcium ion transport
GO:0030154 P:cell differentiation
GO:0007275 P:multicellular organismal development
GO:0007283 P:spermatogenesis
GO:0055085 P:transmembrane transport

References (7)

[1] “A sperm ion channel required for sperm motility and male fertility.”  Ren D.et.al.   11595941
[2] “Human chromosome 11 DNA sequence and analysis including novel gene identification.”  Taylor T.D.et.al.   16554811
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “CatSper gene expression in postnatal development of mouse testis and in subfertile men with deficient sperm motility.”  Nikpoor P.et.al.   14688170
[5] “The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa.”  Li H.-G.et.al.   16625279
[6] “Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity.”  Zhang D.et.al.   16740636
[7] “Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility.”  Li H.-G.et.al.   17347248

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MDQNSVPEKA QNEADTNNAD RFFRSHSSPP HHRPGHSRAL HHYELHHHGV PHQRGESHHP 
61:	PEFQDFHDQA LSSHVHQSHH HSEARNHGRA HGPTGFGLAP SQGAVPSHRS YGEDYHDELQ 
121:	RDGRRHHDGS QYGGFHQQSD SHYHRGSHHG RPQYLGENLS HYSSGVPHHG EASHHGGSYL 
181:	PHGPNPYSES FHHSEASHLS GLQHDESQHH QVPHRGWPHH HQVHHHGRSR HHEAHQHGKS 
241:	PHHGETISPH SSVGSYQRGI SDYHSEYHQG DHHPSEYHHG DHPHHTQHHY HQTHRHRDYH 
301:	QHQDHHGAYH SSYLHGDYVQ STSQLSIPHT SRSLIHDAPG PAASRTGVFP YHVAHPRGSA 
361:	HSMTRSSSTI RSRVTQMSKK VHTQDISTKH SEDWGKEEGQ FQKRKTGRLQ RTRKKGHSTN 
421:	LFQWLWEKLT FLIQGFREMI RNLTQSLAFE TFIFFVVCLN TVMLVAQTFA EVEIRGEWYF 
481:	MALDSIFFCI YVVEALLKII ALGLSYFFDF WNNLDFFIMA MAVLDFLLMQ THSFAIYHQS 
541:	LFRILKVFKS LRALRAIRVL RRLSFLTSVQ EVTGTLGQSL PSIAAILILM FTCLFLFSAV 
601:	LRALFRKSDP KRFQNIFTTI FTLFTLLTLD DWSLIYMDSR AQGAWYIIPI LVIYIIIQYF 
661:	IFLNLVITVL VDSFQTALFK GLEKAKQERA ARIQEKLLED SLTELRAAEP KEVASEGTML 
721:	KRLIEKKFGT MTEKQQELLF HYLQLVASVE QEQQKFRSQA AVIDEIVDTT FEAGEEDFRN