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1.A.1.11.36
Two pore segment channel 1 of 790 aas and 12 TMSs in a 3 + 3 (N-terminal half) + 2 + 2 + 2 (C-terminal half) TMS arrangement. In the trunk of developing zebrafish embryos, adjacent myotome blocks transmit contractile force via myoseptal junctions (MJs), dynamic structures that connect the actin cytoskeleton of skeletal muscle cells to extracellular matrix components via transmembrane protein complexes in the sarcolemma. Rice et al. 2022 reported that the endolysosomal ion channel, TPC1, generates highly localized, non-propagating Ca2+ transients that play a distinct and required role in the capture and attachment of superficial slow skeletal muscle cells (SMCs) at MJs. Disruption of the tpcn1 gene resulted in abnormal MJ phenotypes including SMCs detaching from or crossing the myosepta. TPC1-decorated endolysosomes are dynamically associated with MJs in a microtubule-dependent manner, and  attenuating tpcn1 expression or function disrupted endolysosomal trafficking and resulted in an abnormal distribution of beta-dystroglycan (a key transmembrane component of the dystrophin-associated protein complex). Thus, localized TPC1-generated Ca2+ signals facilitate essential endolysosomal trafficking and membrane contact events, which help form and maintain MJs following the onset of SMC contractile activity (Rice et al. 2022).

Accession Number:F1R4Z3
Protein Name:Two pore segment channel 1
Length:790
Molecular Weight:91657.00
Species:Danio rerio (Zebrafish) [7955]
Number of TMSs:12
Substrate calcium(2+)

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FASTA formatted sequence
1:	MKNHLIFFFL LYTDGAGNHD VVNNVTIPAS GASDPRAQNS LQQSWEMNFQ EAAIYLQEGE 
61:	NNDKFFTHPR SAHALSAYLF AHSHLFYVVE LLTAVLLMLL SLSEAPAVPF LRLDVYVHAT 
121:	LELLALVMVA FELCMKLRWL GFHTFIRHKR TMVKTSVLFI QFIEAIVVLV RQTSHLRVTR 
181:	ALRPIFLVDC RYCGAVRRNL RQIFQSLPPF IDILLLLLFF MVIFAILGFC LFSANTADHY 
241:	FSTLENSIVS LFVLLTTANF PDVMMPAYSK NRWSCIFFIV YLSIELYFIM NLLLAVVFDT 
301:	FNGVEKMKFK SLLLHKRSAI EHAFQLLVSR QRPDGVSLKQ FDGLMRFYHP RMTPRDRFLT 
361:	FKALNHSGAP MLSLQDFYKF YEVIGLKWKA RRSGEHWFDD LPRTALLIFK DLVVAVNGIW 
421:	ILVETNMLNG FHSNINFFII IIYLLFMFRW NEVYGIEVLL KITGLGPITY FSSGWNLFDF 
481:	SVTVFAFLGL IALAFDMEPF YFIVVLRPLQ LLRLFKIKQR YRNVLDTMFE LFPRMASLGL 
541:	TLIIFYYSFA IVGMEFFADV IYPNCCDNST VAASYRQINV TTGNRTELQE GYYYLNNFNN 
601:	ILSSFVTLFE LTVVNNWYIT MEGVTSQTTH WSRLYFMTFY IVTMVVMTII VAFILDAFVF 
661:	RMNYSRKNRE PVTGTFFTEI SNTESLCGIV FETEVSQTEA LQTLDFYKHT LGVTNLTSLQ 
721:	DMCVALERSG QTSLVFLGRR SRTKSDLSMK MYEEEIQEWY EEYSRTNLQT DGTFHRELDS 
781:	PDAGATNSIN