TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.1.5.17
Cyclic nucleotide-gated K+channel of 430 aas, probably with 6 TMSs in a 2 + 2 + 1 + P-loop + 1 TMS arrangement.  The channel is activated by cAMP, not by cGMP, and is highly specific for K+ over Na+.  It has a C-terminal hydrophilic cAMP-binding domain linked to the 6 TMS channel domain (Brams et al. 2014). An SthK C-linker domain is essential for coupling cyclic nucleotide binding to channel opening (Evans et al. 2020). An agonist-dependent conformational change in which residues of the B'-helix displayed outward movement with respect to the symmetry axis of the channel in the presence of cAMP was observed, but not with the partial agonist, cGMP. This conformational rearrangement was observed both in detergent-solubilized SthK and in channels reconstituted into lipid nanodiscs. In addition to outward movement of the B'-helix, channel activation involves upward translation of the cytoplasmic domain with formation of state-dependent interactions between the C-linker and the transmembrane domain (Evans et al. 2020).

Accession Number:G0GA88
Protein Name:Putative transcriptional regulator, Crp/Fnr family
Length:430
Molecular Weight:48269.00
Species:Spirochaeta thermophila (strain ATCC 700085 / DSM 6578 / Z-1203) [869211]
Number of TMSs:3
Substrate K+

Cross database links:

Structure:
6CJQ   6CJT   6CJU     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MKSSGVSNPT YTLVWKVWIL AVTLYYAIRI PLTLVFPSLF SPLLPLDILA SLALIADIPL 
61:	DLAFESRRTS GRKPTLLAPS RLPDLLAALP LDLLVFALHL PSPLSLLSLV RLLKLISVQR 
121:	SATRILSYRI NPALLRLLSL VGFILLAAHG IACGWMSLQP PSENPAGTRY LSAFYWTITT 
181:	LTTIGYGDIT PSTPTQTVYT IVIELLGAAM YGLVIGNIAS LVSKLDAAKL LHRERVERVT 
241:	AFLSYKRISP ELQRRIIEYF DYLWETRRGY EEREVLKELP HPLRLAVAME IHGDVIEKVP 
301:	LFKGAGEEFI RDIILHLEPV IYGPGEYIIR AGEMGSDVYF INRGSVEVLS ADEKTRYAIL 
361:	SEGQFFGEMA LILRAPRTAT VRARAFCDLY RLDKETFDRI LSRYPEIAAQ IQELAVRRKE 
421:	ELEGGTSRRG