1.A.23.4.14
Mechanosensitive channel-like 10, Msl10 of 734 aas and 5 or more TMSs.  It functions in triggering cell death in a process that is independent of its channel activity (Maksaev et al. 2018). The N-terminus of MSL10 (MSL10(N)) is an exemple of these IDRs. MSL10(N) adopted a predominately helical structure when exposed to the helix-inducing compound, trifluoroethanol (TFE), but in the presence of molecular crowding agents, MSL10(N) underwent structural changes and exhibited alterations to its homotypic interaction favorability. Collective behavior via in vitro imaging of condensates indicated that MSL8(N), MSL9(N), and MSL10(N) have sharply differing propensities for self-assembly into condensates, both inherently and in response to salt, temperature, and molecular crowding. These data establish the N-termini of MSL channels as IDRs with distinct biophysical properties and the potential to respond uniquely to changes in their physiochemical environments (Flynn et al. 2023).