1.A.23.4.16
MscA, a mechanosensitive channel in the ER membranes of filamentous fungi (AN7571). It may have 6 or 7 TMSs in a 4 + 2 or 3 TMS arrangement, but there are also two moderately hydrophobic peaks near the C-terminus of the protein that might be TMSs. Orthologues of MscA and MscB are present in most fungi, including plant and animal pathogens. MscA/MscB and other fungal MscS-like proteins share the three TMSs and the extended C-terminal cytosolic domain that form the structural fingerprint of MscS-like channels (Dionysopoulou et al. 2022). Their overexpression leads to increased CaCl2 toxicity or/and reduction of asexual spore formation.
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| Accession Number: | Q5AVV9 |
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| Protein Name: | Mechanosensitive ion channel protein |
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| Length: | 944 |
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| Molecular Weight: | 105160.00 |
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| Species: | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [227321] |
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| Number of TMSs: | 6 |
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| Location1 / Topology2 / Orientation3: |
Endoplasmic reticulum membrane1 |
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| Substrate |
ion |
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1: MTTPVNEVPE PSYPAAERRR RSDSDSSPTV DGNAYVHDES QPHDGAYAGA QYGSHLQVDT
61: TFQDFDKTSR SPSAAREQAM RLEDDLAVLE AERVASRSTH GTEKDARSGD VHSLTRSRSR
121: RAEDVDEFDE ATNPLHEKAA VYNPPENPST GVSKFIKRVH ESSFIVRYFT YIVPLVLILL
181: IPLLVGALAY PDASVGGVEL LWFSVWLEIV WLTLWAGRIV AKLLPTPVNI FASIFTNNSK
241: KWRDVAKQLE LPVTLFLWWL GVEISFLPTM KNHHVDGNSR TRDWENTLNK IIISVFVWTI
301: LNLIEKFLLQ LIAMSFHRRT YSDRIEINKF QIGSLTKLYA FSRNKIAETD EAFEEKQDKS
361: GSGAKTPLRY AGKARGLALG ALNKVGDVAG AVAADFTGRK ANSSSHPSQV VIALLRTTAG
421: CQTLARRLYR TFVREGFDTV FPGDLKEAFD DNEEAEAAFS MFDKDMNGDI SMEELEAVCV
481: EIGRERKAIT ASLKDLDSVV SRLGNVFEFF VAVIAIIVFL TLISTSAAGV LTSAGSSILA
541: LSWLFSATAQ EFLQSVVFVF VKHPFDVGDR VTIYGNSGEA GLGDDYYVKQ ISLLYTEFKK
601: MQGHIVQAPN SYLNTLFVLN QRRSGALAEA VPVVIKYGTT LEQMDALRQR LLEFVRSERR
661: EFQTNILTEL RAVTENFSVT LNVVFFYKSN WQNEGLRLQR RNKFICMLMV ALQEIGIEGP
721: RMNLQGASVD IPYHIAGFPP PYQARDGPPV NDDGGQHPPH TPSSGDDQHH QPGHSPSYRR
781: PSILRKGMVA ANARARGVSI SSRKHVDFSL GMRDVASNDI MGDVFEARSP AADSAVRQVI
841: AERRIQEEEA ERQSVESSRQ RNSGSRTNLT VPTSTNEARH SIDSQGASSL SSISRNRFFR
901: HRSHSHSTRQ EDLMEQGRSS EQGSSSAAAI RPISPSYHPD EKKL