TCDB is operated by the Saier Lab Bioinformatics Group

1.B.38 The Treponema Porin Major Surface Protein (TP-MSP) Family

MSP is a 53 kDa (474 aa) surface antigen in the outer sheath of Treponema denticola. It is an adhesin, but additionally it has been purified to homogeneity and reconstituted in black lipid membranes where it showed channel activity (Egli et al., 1993). It also induces channel activity in HeLa cell membranes (Mathers et al., 1996). The channel had a single conductance of 1.8 nS in 0.1 M KCl (estimated pore diameter of 3.4 nm), the largest porin channel documented in 1996. Electron microscopy suggested a regular hexagonal array in the membrane. Homologues are found in other Treponema species.

The transport reaction catalyzed by MSP is:

ions (non-selective)(periplasm) ions (out)

References associated with 1.B.38 family:

Anand A., LeDoyt M., Karanian C., Luthra A., Koszelak-Rosenblum M., Malkowski MG., Puthenveetil R., Vinogradova O. and Radolf JD. (2015). Bipartite Topology of Treponema pallidum Repeat Proteins C/D and I: OUTER MEMBRANE INSERTION, TRIMERIZATION, AND PORIN FUNCTION REQUIRE A C-TERMINAL beta-BARREL DOMAIN. J Biol Chem. 290(19):12313-31. 25805501
Anand, A., A. Luthra, M.E. Edmond, M. Ledoyt, M.J. Caimano, and J.D. Radolf. (2013). The major outer sheath protein (Msp) of Treponema denticola has a bipartite domain architecture and exists as periplasmic and outer membrane-spanning conformers. J. Bacteriol. 195: 2060-2071. 23457251
Cameron, E.A., M.A. Maynard, C.J. Smith, T.J. Smith, N.M. Koropatkin, and E.C. Martens. (2012). Multidomain Carbohydrate-binding Proteins Involved in Bacteroides thetaiotaomicron Starch Metabolism. J. Biol. Chem. 287: 34614-34625. 22910908
Cho, K.H. and A.A. Salyers. (2001). Biochemical analysis of interactions between outer membrane proteins that contribute to starch utilization by Bacteroides thetaiotaomicron. J. Bacteriol. 183: 7224-7230. 11717282
Egli, C., W.K. Leung, K.H. Muller, R.E. Hancock, and B.C. McBride. (1993). Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola. Infect. Immun. 61: 1694-1699. 7682993
Foley, M.H., E.C. Martens, and N.M. Koropatkin. (2018). SusE facilitates starch uptake independent of starch binding in B. thetaiotaomicron. Mol. Microbiol. 108: 551-566. 29528148
Giacani, L., S.L. Brandt, M. Puray-Chavez, T.B. Reid, C. Godornes, B.J. Molini, M. Benzler, J.S. Hartig, S.A. Lukehart, and A. Centurion-Lara. (2012). Comparative investigation of the genomic regions involved in antigenic variation of the TprK antigen among treponemal species, subspecies, and strains. J. Bacteriol. 194: 4208-4225. 22661689
Joglekar, P., E.D. Sonnenburg, S.K. Higginbottom, K.A. Earle, C. Morland, S. Shapiro-Ward, D.N. Bolam, and J.L. Sonnenburg. (2018). Genetic Variation of the SusC/SusD Homologs from a Polysaccharide Utilization Locus Underlies Divergent Fructan Specificities and Functional Adaptation in Strains. mSphere 3:. 29794055
Kumar, S., M.J. Caimano, A. Anand, A. Dey, K.L. Hawley, M.E. LeDoyt, C.J. La Vake, A.R. Cruz, L.G. Ramirez, L. Paštěková, I. Bezsonova, D. Šmajs, J.C. Salazar, and J.D. Radolf. (2018). Sequence Variation of Rare Outer Membrane Protein β-Barrel Domains in Clinical Strains Provides Insights into the Evolution of subsp. , the Syphilis Spirochete. MBio 9:. 29895642
Mathers, D.A., W.K. Leung, J.C. Fenno, Y. Hong, and B.C. McBride. (1996). The major surface protein complex of Treponema denticola depolarizes and induces ion channels in HeLa cell membranes. Infect. Immun. 64: 2904-2910. 8757811
Puthenveetil, R., S. Kumar, M.J. Caimano, A. Dey, A. Anand, O. Vinogradova, and J.D. Radolf. (2017). The major outer sheath protein forms distinct conformers and multimeric complexes in the outer membrane and periplasm of Treponema denticola. Sci Rep 7: 13260. 29038532
Reid, T.B., B.J. Molini, M.C. Fernandez, and S.A. Lukehart. (2014). Antigenic variation of TprK facilitates development of secondary syphilis. Infect. Immun. 82: 4959-4967. 25225245
Shipman, J.A., J.E. Berleman, and A.A. Salyers. (2000). Characterization of four outer membrane proteins involved in binding starch to the cell surface of Bacteroides thetaiotaomicron. J. Bacteriol. 182: 5365-5372. 10986238