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2.A.31.2.1
Electroneutral Na+:HCO3- cotransporter (NBCn1 or NBC2 or Slc4a7) of 1218 aas and 11 or 12 TMSs. Sodium- and bicarbonate-dependent cotransporter with a Na+:HCO3- = 1:1 stoichiometry (Praetorius et al. 2004). It mediates transport important for pH recovery after acid load as well as for regulation of steady-state pH in the duodenum and vascular smooth muscle cells, and plays a key role in macrophage acidification, mediating bicarbonate import into the cytoplasm which is crucial for net acid extrusion and maintenance of cytoplasmic pH during phagocytosis.  It also
provides cellular bicarbonate for de novo purine and pyrimidine synthesis and is a key mediator of de novo nucleotide synthesis downstream of mTORC1 signaling in proliferating cells. NBCn1 localizes to the plasma membrane, centrosomes, spindle and primary cilia (Severin et al. 2023).

Accession Number:Q9R1N3
Protein Name:NBCn1-D aka NBC2
Length:1218
Molecular Weight:135954.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Basolateral cell membrane1 / Multi-pass membrane protein2
Substrate sodium(1+), hydrogencarbonate

Cross database links:

RefSeq: NP_478118.1   
Entrez Gene ID: 117955   
Pfam: PF07565    PF00955   
KEGG: rno:117955   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0016323 C:basolateral plasma membrane
GO:0031410 C:cytoplasmic vesicle
GO:0016021 C:integral to membrane
GO:0032420 C:stereocilium
GO:0005452 F:inorganic anion exchanger activity
GO:0015293 F:symporter activity
GO:0006820 P:anion transport
GO:0006814 P:sodium ion transport

References (10)

[1] “An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel.”  Choi I.et.al.   10850716
[2] “Immunoelectron microscopic localization of NBC3 sodium-bicarbonate cotransporter in rat kidney.”  Kwon T.-H.et.al.   10662737
[3] “Immunolocalization of electroneutral Na-HCO(3)(-) cotransporter in rat kidney.”  Vorum H.et.al.   11053051
[4] “Immunolocalization of NBC3 and NHE3 in the rat epididymis: colocalization of NBC3 and the vacuolar H+-ATPase.”  Pushkin A.et.al.   10975418
[5] “The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3.”  Park M.et.al.   12403779
[6] “NBCn1 is a basolateral Na+-HCO3- cotransporter in rat kidney inner medullary collecting ducts.”  Praetorius J.et.al.   15075186
[7] “Basolateral Na+-dependent HCO3- transporter NBCn1-mediated HCO3-influx in rat medullary thick ascending limb.”  Odgaard E.et.al.   14673192
[8] “Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2.”  Reiners J.et.al.   16301216
[9] “Molecular and functional characterization of the electroneutral Na/HCO3 cotransporter NBCn1 in rat hippocampal neurons.”  Cooper D.S.et.al.   15718246
[10] “An anti-NH2-terminal antibody localizes NBCn1 to heart endothelia and skeletal and vascular smooth muscle cells.”  Damkier H.H.et.al.   16126812

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MEADGAGEQM RPLLTRGPDE EAVVDLGKTS STVNTKFEKE ELESHRAVYV GVHVPFSKES 
61:	RRRHRHRGHK HHHRRRKDKD SDKEDGRESP SYDTPSQRVQ FILGTEDDDE EHIPHDLFTE 
121:	MDELCYRDGE EYEWKETARW LKFEEDVEDG GDRWSKPYVA TLSLHSLFEL RSCILNGTVM 
181:	LDMRASTLDE IADMVLDNMI ASGQLDDSIR ENVREALLKR HHHQNEKRFT SRIPLVRSFA 
241:	DIGKKHSDPH LLERNGEGLS ASRHSLRTGL SASNLSLRGE SPLSLLLSHL LPSSRAGTPA 
301:	GSRCTTPVPT PQNSPPSSPS LSRLTSRSSQ QTQPQAPEVL VSPDRDDIPR VVIHPPEEDI 
361:	EALKGQEQKN EENTDFTPGI LASPQSAPGN LDSSKSGEVK GNGSGGSREN STVDFSKVDM 
421:	NFMRKIPTGA EASNVLVGEV DFLERPIIAF VRLAPAVLLS GLTEVPVPTR FLFLLLGPAG 
481:	KAPQYHEIGR SIATLMTDEI FHDVAYKAKD RNDLLSGIDE FLDQVTVLPP GEWDPSIRIE 
541:	PPKSVPSQEK RKIPAFPNGS APVSADPPKE ADHHAGPELQ RTGRLFGGLI LDIKRKAPFF 
601:	LSDFKDALSL QCLASILFLY CACMSPVITF GGLLGEATEG RISAIESLFG ASLTGIAYSL 
661:	FAGQPLTILG STGPVLVFEK ILFKFCRDYH LSYLSLRTSI GLWTSFLCIV LVATDASSLV 
721:	CYITRFTEEA FAALICIIFI YEALEKLFHL GEIYAFNMHN NLDALTSYTC VCAEPSNPSN 
781:	ETVELWERKN VTAASISWAN LTVSECKTFH GMFVGSACGP HGPYVPDVLF WCVVLFFTTF 
841:	FLSSFLKQFK TKRYFPTKVR STISDFAVFL TIVIMVAIDY LVGIPSPKLH VPEKFEPTDP 
901:	SRGWIISPLG DNPWWTLLIA AVPALLCTIL IFMDQQITAV IINRKEHKLK KGAGYHLDLL 
961:	MVAVMLGVCS IMGLPWFVAA TVLSISHVNS LKVESECSAP GEQPKFLGIR EQRVTGLMIF 
1021:	ILMGLSVFMT SVLKFIPMPV LYGVFLYMGV SSLKGIQFFD RIKLFGMPAK HQPDLIYLRY 
1081:	VPLWKVHVFT VVQLTCLVLL WVIKASAAAV VFPMMVLALV FVRKLMDLCF TKRELSWLDD 
1141:	LMPESKKKKE DDKKKKEKEE AERMLQGDGD TVHLPFERGS LLQIPVKTLK YSPEKPVSVT 
1201:	INFEDEPSKK YMDAETSL