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2.A.4.3.4
Plant root and leaf vacuolar Zn2+ transporter, ZAT-1 or MTP1 (metal tolerance protein 1) (Desbrosses-Fonrouge et al., 2005). Loss of the cytoplasmic histidine-rich loop, where 4 Zn2+ (or Ni2+ or Co2+) bind, stimulates transport activity (Kawachi et al., 2008), presumably providing a feedback sensor of cytoplasmic Zn2+ (Tanaka et al. 2013). The barley orthologue has broader metal specificity (Podar et al., 2012). Critical residues for function, ion selectivity and structure have been identified (Kawachi et al., 2012). In Citrus sinensis, expression is induced by Cd2+ (Fu et al. 2017).

Accession Number:Q9ZT63
Protein Name:Metal tolerance protein 1 aka ZAT1p
Length:398
Molecular Weight:43827.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate zinc(2+)

Cross database links:

RefSeq: NP_182203.1    NP_850459.1   
Entrez Gene ID: 819293   
Pfam: PF01545   
KEGG: ath:AT2G46800   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005774 C:vacuolar membrane
GO:0046872 F:metal ion binding
GO:0005385 F:zinc ion transmembrane transporter activity
GO:0006882 P:cellular zinc ion homeostasis
GO:0055085 P:transmembrane transport
GO:0006829 P:zinc ion transport

References (7)

[1] “Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation.”  Van der Zaal B.J.et.al.   10069843
[2] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[3] “Characterization of the ZAT1p zinc transporter from Arabidopsis thaliana in microbial model organisms and reconstituted proteoliposomes.”  Bloss T.et.al.   11882948
[4] “Phylogenetic relationships within cation transporter families of Arabidopsis.”  Maeser P.et.al.   11500563
[5] “Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.”  Shimaoka T.et.al.   15215502
[6] “Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis.”  Kobae Y.et.al.   15653794
[7] “MTP1 mops up excess zinc in Arabidopsis cells.”  Kraemer U.et.al.   15946891

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MESSSPHHSH IVEVNVGKSD EERIIVASKV CGEAPCGFSD SKNASGDAHE RSASMRKLCI 
61:	AVVLCLVFMS VEVVGGIKAN SLAILTDAAH LLSDVAAFAI SLFSLWAAGW EATPRQTYGF 
121:	FRIEILGALV SIQLIWLLTG ILVYEAIIRI VTETSEVNGF LMFLVAAFGL VVNIIMAVLL 
181:	GHDHGHSHGH GHGHGHDHHN HSHGVTVTTH HHHHDHEHGH SHGHGEDKHH AHGDVTEQLL 
241:	DKSKTQVAAK EKRKRNINLQ GAYLHVLGDS IQSVGVMIGG AIIWYNPEWK IVDLICTLAF 
301:	SVIVLGTTIN MIRNILEVLM ESTPREIDAT KLEKGLLEME EVVAVHELHI WAITVGKVLL 
361:	ACHVNIRPEA DADMVLNKVI DYIRREYNIS HVTIQIER