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2.A.40.3.3 Uric acid (urate) permease YgfU. YgfU exhibits low affinity (0.5 mM) but high capacity for urate and very poor activity for xanthine. Essential residues were identified. Coversion of Thr-100 to ala resulted in efficient xanthine transport (Papakostas and Frillingos 2012).
Accession Number:	Q46821
Protein Name:	Putative purine permease ygfU
Length:	482
Molecular Weight:	51758.00
Species:	Escherichia coli (strain K12) [83333]
Number of TMSs:	12
Location¹ / Topology² / Orientation³:	Cell inner membrane¹ / Multi-pass membrane protein²
Substrate	7,9-dihydro-1H-purine-2,6,8(3H)-trione, 9H-xanthine

DIP:	DIP-12176N
Entrez Gene ID:	949017
Pfam:	PF00860
KEGG:	eco:b2888
Gene Ontology GO:0016021 C:integral to membrane GO:0005886 C:plasma membrane GO:0005215 F:transporter activity GO:0055085 P:transmembrane transport
References (3) [1] “The complete genome sequence of Escherichia coli K-12.” Blattner F.R.et.al. 9278503 [2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.” Hayashi K.et.al. 16738553 [3] “Global topology analysis of the Escherichia coli inner membrane proteome.” Daley D.O.et.al. 15919996

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FASTA formatted sequence

1:	MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL MIGDRLGLSK 
61:	EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT FAAVTPMIAI GMNPDIGLLG 
121:	IFGATIAAGF ITTLLAPLIG RLMPLFPPLV TGVVITSIGL SIIQVGIDWA AGGKGNPQYG 
181:	NPVYLGISFA VLIFILLITR YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA 
241:	IVTPMSFGMP IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV 
301:	GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM AVLVASIPQF 
361:	VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL GVGMTPTLSH DFFSKLPAVL 
421:	QPLLHSGIML ATLSAVVLNV FFNGYQHHAD LVKESVSDKD LKVRTVRMWL LMRKLKKNEH 
481:	GE

2.A.40.3.3
Uric acid (urate) permease YgfU. YgfU exhibits low affinity (0.5 mM) but high capacity for urate and very poor activity for xanthine. Essential residues were identified. Coversion of Thr-100 to ala resulted in efficient xanthine transport (Papakostas and Frillingos 2012).

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Gene Ontology

References (3)

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2.A.40.3.3 Uric acid (urate) permease YgfU. YgfU exhibits low affinity (0.5 mM) but high capacity for urate and very poor activity for xanthine. Essential residues were identified. Coversion of Thr-100 to ala resulted in efficient xanthine transport (Papakostas and Frillingos 2012).

Cross database links:

Gene Ontology

References (3)

External Searches:

Analyze:

2.A.40.3.3
Uric acid (urate) permease YgfU. YgfU exhibits low affinity (0.5 mM) but high capacity for urate and very poor activity for xanthine. Essential residues were identified. Coversion of Thr-100 to ala resulted in efficient xanthine transport (Papakostas and Frillingos 2012).