2.A.53.1.14 Sulfate transporter 4:1, chloroplastic, SULTR4;1, MSH12.1, of 685 aas and 13 - 15 TMSs. It is a H+/sulfate cotransporter that may play a role in the regulation of sulfate assimilation. The structure of AtSULTR4;1, in complex with SO42- at an overall resolution of 2.8 Å has been determined (Wang et al. 2021). AtSULTR4;1 forms a homodimer and has a structural fold typical of the SLC26 family of anion transporters. The bound SO42- is coordinated by side-chain hydroxyls and backbone amides, and further stabilized electrostatically by the conserved Arg393 and two helix dipoles. Proton and SO42- are co-transported, and a proton gradient significantly enhances SO42- transport. Glu347, which is ~7 Å from the bound SO42-, is required for H+-driven transport. The cytosolic STAS domain interacts with the transmembrane domains, and deletion of the STAS domain, or mutations at the interface, compromise dimer formation and reduces SO42- transport, suggesting a regulatory function of the STAS domain (Wang et al. 2021).
|
Accession Number: | Q9FY46 |
Protein Name: | Sulfate transporter 4.1, chloroplastic |
Length: | 685 |
Molecular Weight: | 75096.00 |
Species: | Arabidopsis thaliana (Mouse-ear cress) [3702] |
Number of TMSs: | 13 |
Location1 / Topology2 / Orientation3: |
Plastid1 / Multi-pass membrane protein2 |
Substrate |
sulfate |
---|
1: MSYASLSVKD LTSLVSRSGT GSSSSLKPPG QTRPVKVIPL QHPDTSNEAR PPSIPFDDIF
61: SGWTAKIKRM RLVDWIDTLF PCFRWIRTYR WSEYFKLDLM AGITVGIMLV PQAMSYAKLA
121: GLPPIYGLYS SFVPVFVYAI FGSSRQLAIG PVALVSLLVS NALGGIADTN EELHIELAIL
181: LALLVGILEC IMGLLRLGWL IRFISHSVIS GFTSASAIVI GLSQIKYFLG YSIARSSKIV
241: PIVESIIAGA DKFQWPPFVM GSLILVILQV MKHVGKAKKE LQFLRAAAPL TGIVLGTTIA
301: KVFHPPSISL VGEIPQGLPT FSFPRSFDHA KTLLPTSALI TGVAILESVG IAKALAAKNR
361: YELDSNSELF GLGVANILGS LFSAYPATGS FSRSAVNNES EAKTGLSGLI TGIIIGCSLL
421: FLTPMFKYIP QCALAAIVIS AVSGLVDYDE AIFLWRVDKR DFSLWTITST ITLFFGIEIG
481: VLVGVGFSLA FVIHESANPH IAVLGRLPGT TVYRNIKQYP EAYTYNGIVI VRIDSPIYFA
541: NISYIKDRLR EYEVAVDKYT NRGLEVDRIN FVILEMSPVT HIDSSAVEAL KELYQEYKTR
601: DIQLAISNPN KDVHLTIARS GMVELVGKEW FFVRVHDAVQ VCLQYVQSSN LEDKHLSFTR
661: RYGGSNNNSS SSNALLKEPL LSVEK