Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.53.3.10
Vacuolar membrane protein, YGR125W, basic amino acid (arginine, etc.) transporter of 1036 aas and 10 - 13 TMSs (Kawano-Kawada et al. 2021). The membrane domain is found in the central part of the protein, residues 210 - 630 with large hydrophilic N- and C-terminal domains. The large C-terminal domain may be a PE-PGRS family domain, some of which may be outer membrane porins (see TC# 9.B.96), and there may be an additional TMS at the C-terminus. Vacuolar levels of basic amino acids drastically decrease in Δygr125w cells. An expression plasmid of YGR125w with HA3-tag inserted in its N-terminal hydrophilic region restored the vacuolar levels of basic amino acids. Uptake of arginine into vacuolar membrane vesicles depended on HA3-YGR125w expression. A conserved aspartate residue in the first TMS (D223) was indispensable for the accumulation of basic amino acids. YGR125w is also designated VSB1. Thus, Ygr125w/Vsb1 contributes to (1) the uptake of arginine into vacuoles and (2) vacuolar compartmentalization of basic amino acids (Kawano-Kawada et al. 2021).

Accession Number:P53273
Protein Name:Uncharacterized vacuolar membrane protein YGR125W
Length:1036
Molecular Weight:116971.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate L-lysine, ornithine, arginine, histidine

Cross database links:

DIP: DIP-7435N
Entrez Gene ID: 853026   
Pfam: PF00027    PF01740    PF00916   
KEGG: sce:YGR125W   

Gene Ontology

GO:0000324 C:fungal-type vacuole
GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0005215 F:transporter activity
GO:0055085 P:transmembrane transport

References (9)

[1] “An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes.”  van Dyck L.et.al.   9046098
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.”  Tettelin H.et.al.   9169869
[3] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[4] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[5] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[7] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[8] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[9] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MGRTIRRRRS NSSLSEAISV SLGINQDSSV NKMHRASVSA MSPPLCRSYM SGFFTGGNSP 
61:	MINNLSDSKL PISNKQHPKV IHGSENLHRQ TAQLSNEFCS SSVEENSPTI KDYMDIIGNG 
121:	DRKDDQSMRT IEENIDEEYS DEYSRLLLSP ASSNVDDDRN RGLQNSSLPE LEDGYAGGYQ 
181:	SLRPSHNLRF RPRNLWHMCT SFPSKFAHYL PAAVLGLLLN ILDALSYGMI IFPITEPVFS 
241:	HLGPTGISMF YISTIISQAV YSGGWSSFPS GIGSEMIEIT PFYHTMALAI KEALAGNDDE 
301:	IITTTIFCYV ISSMLTGVVF YALGKLRLGK IVGFFPRHIL IGCIGGVGYF LIITGIEVTT 
361:	RVAKFEYSWP FFSGLFTDYD TLAKWLLPVL LTVVLIGTQR YFKNSLVLPS FYILTLVLFH 
421:	FIVAIIPTLS LDALRQAGWI FPIANSDSKW YDHYRLFNVH KVHWSLVLQQ IPTMMALTFF 
481:	GILHVPINVP ALAMSLQMDK YDVDRELIAH GYSNFFSGLL GSVQNYLVYT NSVLFIRAGA 
541:	DSPFAGFLLI ALTICIMIIG PVIISFIPIC IVGSLIFLLG YELLVEALVD TWNKLNRFEY 
601:	LTVVIIVFTM GIFDFVLGII VGILIACFSF LVDSTKLQTI NGEYNGNVAR STVYRDYVQT 
661:	KFLDGIGEQI YVLKLQNLLF FGTIISIEEK IERLLQISNK DATKRRIKYL ILDFKNINAD 
721:	NIDYSAAEGF NRIKRFTETK RIKLIISSIK ERDRIYNAFN NVGLLNDVEL FADLNSALEW 
781:	CENEFLFQYK QLRKKAKERL EEGKQNNVVS AVIAATKNKK IDTIGNGLNR GSNGDTARNL 
841:	MSLPTNTPRN YQILSVAQNV FVNDEQAVKN FKKEYKDDEP VLPILLFALK QYRPDIISEV 
901:	QKVREKEIKF WAQLCPYFTR RRLASQSHLL HADNIFFLVE TGMLKATYEL PQGTLYEIFS 
961:	NGTCFGKIIA PGNAMPREQK LTIETETDSV LWVIDSSSLN KLKEDNLALY VEVALMVMCI 
1021:	KDTRFKELLG YTLVSA