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2.A.6.5.2
The lipid exporter, mycobacterial membrane protein Large-7, MmpL7, is required for the export of the virulence lipid, phthiocerol dimycocerosate (PDIM), in Mycobacterium tuberculosis. MmpL7. It also confers high level isoniazid efflux and resistance (Pasca et al., 2005). MmpL7 is also involved in the transport of the structurally related phenolic glycolipid (PGL). Moolla et al. 2021 generated an in silico model of MmpL7 that revealed MmpL7 as a functional outlier within the MmpL family, missing a canonical proton-relay signature sequence, suggesting that it employs a yet-unidentified mechanism for energy coupling for transport. The periplasmic porter domain 2 insert (PD2-insert), which doesn't share recognisable homology, is highly alpha-helical in nature, suggesting an organisation similar to that seen in the hopanoid PD3/4 domains. They also identified residues present in the transmembrane domains TM4 and TM10, and the PD2 domain insert that play crucial roles in PDIM transport (Moolla et al. 2021).  

Accession Number:P65370
Protein Name:Putative membrane protein MML7 aka MmpL7 aka RV2942 aka MT3012 aka MTCY24G1.07C
Length:920
Molecular Weight:95122.00
Species:Mycobacterium tuberculosis [1773]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate isoniazide

Cross database links:

RefSeq: NP_217458.1    NP_337525.1   
Entrez Gene ID: 887548    925253   
KEGG: mtc:MT3012    mtu:Rv2942   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane

References (2)

[1] “Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.”  Cole S.T.et.al.   9634230
[2] “Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains.”  Fleischmann R.D.et.al.   12218036

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPSPAGRLHR IRYIRLKKSS PDCRATITSG SADGQRRSPR LTNLLVVAAW VAAAVIANLL 
61:	LTFTQAEPHD TSPALLPQDA KTAAATSRIA QAFPGTGSNA IAYLVVEGGS TLEPQDQPYY 
121:	DAAVGALRAD TRHVGSVLDW WSDPVTAPLG TSPDGRSATA MVWLRGEAGT TQAAESLDAV 
181:	RSVLRQLPPS EGLRASIVVP AITNDMPMQI TAWQSATIVT VAAVIAVLLL LRARLSVRAA 
241:	AIVLLTADLS LAVAWPLAAV VRGHDWGTDS VFSWTLAAVL TIGTITAATM LAARLGSDAG 
301:	HSAAPTYRDS LPAFALPGAC VAIFTGPLLL ARTPALHGVG TAGLGVFVAL AASLTVLPAL 
361:	IALAGASRQL PAPTTGAGWT GRLSLPVSSA SALGTAAVLA ICMLPIIGMR WGVAENPTRQ 
421:	GGAQVLPGNA LPDVVVIKSA RDLRDPAALI AINQVSHRLV EVPGVRKVES AAWPAGVPWT 
481:	DASLSSAAGR LADQLGQQAG SFVPAVTAIK SMKSIIEQMS GAVDQLDSTV NVTLAGARQA 
541:	QQYLDPMLAA ARNLKNKTTE LSEYLETIHT WIVGFTNCPD DVLCTAMRKV IEPYDIVVTG 
601:	MNELSTGADR ISAISTQTMS ALSSAPRMVA QMRSALAQVR SFVPKLETTI QDAMPQIAQA 
661:	SAMLKNLSAD FADTGEGGFH LSRKDLADPS YRHVRESMFS SDGTATRLFL YSDGQLDLAA 
721:	AARAQQLEIA AGKAMKYGSL VDSQVTVGGA AQIAAAVRDA LIHDAVLLAV ILLTVVALAS 
781:	MWRGAVHGAA VGVGVLASYL AALGVSIALW QHLLDRELNA LVPLVSFAVL ASCGVPYLVA 
841:	GIKAGRIADE ATGARSKGAV SGRGAVAPLA ALGGVFGAGL VLVSGGSFSV LSQIGTVVVL 
901:	GLGVLITVQR AWLPTTPGRR