2.A.66.10.1
Wzx isoprenoid-linked O-antigen precursor glycan translocase. A 12 TMS topology with N- and C-termini in the cytoplasm has been established, and functionally important residues have been identified (Marolda et al. 2010). A substrate:proton antiport mechanism has been established (Islam et al. 2013). The Wzx/Wzy pathway produces repeat-units of mostly 3-8 sugars on the cytosolic face of the cytoplasmic membrane that is translocated by the Wzx flippase to the periplasmic face and is polymerized by Wzy polymerase to give long-chain polysaccharides (Hong et al. 2023).
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| Accession Number: | F1XYT9 |
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| Protein Name: | O-antigen flippase Wzx |
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| Length: | 463 |
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| Molecular Weight: | 51846.00 |
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| Species: | Escherichia coli O157:H7 str. 1125 [997825] |
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| Number of TMSs: | 12 |
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| Substrate |
hydron |
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1: MIMNKIKKIL KFCTLKKYDT SSALGREQER YRIISLSVIS SLISKILSLL SLILTVSLTL
61: PYLGQERFGV WMTITSLGAA LTFLDLGIGN ALTNRIAHSF ACGKNLKMSR QISGGLTLLA
121: GLSFVITAIC YITSGMIDWQ LVIKGINENV YAELQHSIKV FVIIFGLGIY SNGVQKVYMG
181: IQKAYISNIV NAIFILLSII TLVISSKLHA GLPVLIVSTL GIQYISGIYL TINLIIKRLI
241: KFTKVNIHAK REAPYLILNG FFFFILQLGT LATWSGDNFI ISITLGVTYV AVFSITQRLF
301: QISTVPLTIY NIPLWAAYAD AHARNDTQFI KKTLRTSLKI VGISSFLLAF ILVVFGSEVV
361: NIWTEGKIQV PRTFIIAYAL WSVIDAFSNT FASFLNGLNI VKQQMLAVVT LILIAIPAKY
421: IIVSHFGLTV MLYCFIFIYI VNYFIWYKCS FKKHIDRQLN IRG