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2.A.99.1.2
The 6TMS Ni2+ uptake porter, HupE2 (Brito et al., 2010).

Accession Number:Q1M8G8
Protein Name:Putative transmembrane hydrogenase-related protein
Length:201
Molecular Weight:19766.00
Species:Rhizobium leguminosarum bv. viciae (strain 3841) [216596]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Golgi apparatus membrane1 / Multi-pass membrane protein2
Substrate nickel(2+)

Cross database links:

RefSeq: YP_765560.1   
Entrez Gene ID: 4398696   
KEGG: rle:pRL90274   

Gene Ontology

GO:0016021 C:integral to membrane

References (1)

[1] “The genome of Rhizobium leguminosarum has recognizable core and accessory components.”  Young J.P.W.et.al.   16640791

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKSALKSGLL ALAAAALPAV AYAHPAIGEA AGFSHGFAHP ISGLDHVLAM VMVGVFAFQL 
61:	GGRATWLVPT TFVLVMAFGG ALGAAGINVP FVETGIALSV VVLGAVVALN VKAPLAAAVG 
121:	LVGLFAIFHG HAHGAEMPEN AAGVAYAAGF MIATALLHVA GLALGYLIGR AGERQGRGIF 
181:	VTRTAGGIAA IAGVGLLAGL I