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3.E.1.7.5
Channel rhodopsin of 829 aas and 7 N-terminal TMSs, KnChR.  Channelrhodopsins (ChRs) are light-gated cation (H+, Na+, K+, and Ca2+) channels extensively applied as optogenetics tools for manipulating neuronal activity. All ChRs have a large cytoplasmic domain. The cation channel properties of KnChR from a filamentous terrestrial alga, Klebsormidium nitens, have been described (Tashiro et al. 2021). The C-terminal cytoplasmic domain has a peptidoglycan binding domain (FimV), and modulates the ion channel properties. The channel closure rate is affected by the C-terminus moiety, and truncation of the moiety to various lengths prolongs the channel open lifetime by more than 10-fold. Two Arginine residues (R287 and R291) are crucial for altering the photocurrent kinetics. Tashiro et al. 2021 proposed that electrostatic interaction between the rhodopsin domain and the C-terminal domain accelerates the channel kinetics. Maximal sensitivity was observed at 430 and 460 nm, the former making KnChR one of the most blue-shifted ChRs, serving as a novel prototype for studying the molecular mechanism of color tuning of the ChRs.

Accession Number:GAQ79757.1
Protein Name:GAQ79757.1 hypothetical protein KFL_000370310 [Klebsormidium nitens]
Length:830
Molecular Weight:
Species:Klebsormidium nitens [105231]
Number of TMSs:7
Substrate

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTVDAHSTVD AHSTVTDSHS AGNGTESCYV ADFLGMHHES HEGALYSVYK SLEWGCFLIS 
61:	IGLFVFYLQQ YRKKTAGWEV IYIAFIESFK YIFEIFWPHN NPAQLNIYGV NKSVPWVRYM 
121:	EWMITCPVIL MALSNISGEE GEYTHRSMQL LATDQGAILC AITAAASEGA ISAVFYAIGV 
181:	CYGICTFYFC LQIYIEAYFT LPETCHSAVK WMAVIFYAGW LCYPCFFLAG SEGWGNLSYE 
241:	GSAIGHCIAD LLSKNAWGVM HWWIRCQLEE YKHTHNGQLP HYSLETRAKM RAEAGHIIAG 
301:	SLGSVLHVAG HQHHHFHFDE DSGMQGAQIQ GQPLAQANER ANLKASLLNG NAVNYNQAVQ 
361:	AQPQAFQIQS GEDGYVISMN QGESSASGSR RTTQNIEGPK KDPVASAFSK ALGSMKSKVT 
421:	EHFTKDLPPS ARPDSPSRRG SARGGADAPT AQPQQSMKID LRDLANNPQL QQQLSMMLKG 
481:	QMAGGYALKT DLKDLANNPQ LQQQLSMMLK GQMGAGNSNT NQGSNNGSPR ARPVSEELAQ 
541:	PARLQKAQSA RATSNLDDEI EEQAPAQMSR RPSARVPRAT AAADEEQAPA RTLSRKISRG 
601:	DGGEPVSEER LQKRPSYRAA KAAAEAADED MTDAPAPALA RRPSARAPAA DADEETAPVR 
661:	TASLRRAGSK SPGNSLPSSP SRRVSRANSL RAPVSASVLL CDADGDMGDF FLDQFAAVPN 
721:	SKCSVKVVAT PDELIDALAT GRAYDFVMVP KGVITSDPEI SEEIRAHAQT PLVAFGPALE 
781:	GMAVAEVKAH MFELDVDDYI GLPKRGQEYP VEDLEALVWK YKKVADDSN