TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
4.D.1.1.1









Lipopolysaccharide glycosyl transferase, WbbF

other sequences
plasmids
WbbF of Salmonella enterica serovar Borreza plasmid pWQ799 (Q52257)
4.D.1.1.2









IcaA of 412 aas and 4 (1 + 3) TMSs.

Bacteria
Firmicutes
IcaA of Staphylococcus epidermidis (Q54066)
4.D.1.1.3









PgaC or YcdQ of 441 aas with a 5 (2 (N-terminal) + 3 (C-terminal)) TMS topology. TC Blast retrieves 4.D.3.1.5 and 4.D.2.1.9 with scores of e-13 and e-8, respectively, for the hydrophilic catalytic domains, demonstrating homology.  This enzyme is an N-acetylglucosaminyltransferase that catalyzes the polymerization of UDP-N-acetylglucosamine to produce the linear homopolymer, poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide (Cerca and Jefferson 2008).  May function with PgaD, a 137 aa, 2 TMS protein (P69432). The polysaccharide is partially deacetylated by PgaB (P75906) in preparation for export across the outer membrane by PgaA (TC# 1.B.55.1.1).

Bacteria
Proteobacteria
PgaC of E. coli (P75905)
4.D.1.1.4









Hyaluronate synthase, HasA

Bacteria
Firmicutes
HasA of Streptococcus pyogenes (P0C0H0)
4.D.1.1.5









The hyaluronan (hyaluronate) synthase/exporter, HAS

Bacteria
Firmicutes
HAS of Streptococcus dysgalactiae subsp. equisimilis (O50201)
4.D.1.1.6









Uncharacterized protein of 354 aas and 4 TMSs.

Bacteria
Proteobacteria
UP of Beggiatoa sp
4.D.1.1.7









Alginate synthesis-related protein of 882 aas and 5 TMSs
Bacteria
Aquificae
Alginate synthesis-related protein of Aquifex aeolicus
4.D.1.1.8









Synthase of type 3 pneumoclccal capsular polysaccharide of 417 aas and 4 TMSs, Cap3B.

Bacteria
Firmicutes
Cap3B of Streptococcus pneumoniae
4.D.1.1.9









Glycosyl transferase involved in alginate biosynthesis of 494 aas and ~ 5 TMSs, Alg8 (Fata Moradali et al. 2015).

Bacteria
Proteobacteria
Alg8 of Pseudomonas aeruginosa
4.D.1.1.10









The hyaluronan (hyaluronate) synthase1/exporter, HAS1

Eukaryota
Metazoa
HAS1 of Homo sapiens (Q92839)
4.D.1.1.11









Uncharacterized protein, YaiP, of 398 aas with 1 N-terminal TMS and 3 C-terminal TMSs.

Bacteria
Proteobacteria
YaiP of E. coli
4.D.1.1.12









Chitin synthase 3, Chs3, of 1165 aas and 7 TMSs in a 2 + 1 + 4 TMS arrangement. Chitin biosynthesis in yeast is accomplished by three chitin synthases (Chs) termed Chs1 (TC# 4.D.1.1.17), Chs2 (TC# 4.D.1.1.16) and Chs3, the last which accounts for most of the chitin deposited within the cell wall. While the overall structures of Chs1 and Chs2 are similar to those of other chitin synthases from fungi and arthropods, Chs3 lacks some of the C-terminal transmembrane helices raising questions regarding its structure and topology. Gohlke et al. 2017 determined aspects of the catalytic domain, the chitin-translocating channel and the interfacial helices in between. They identified an amphipathic, crescent-shaped alpha-helix attached to the inner side of the membrane that may control the channel entrance and a finger helix pushing the polymer into the channel. Chitin synthases form oligomeric complexes, which may be necessary for the formation of chitin nanofibrils. They detected oligomeric complexes at the bud neck, the lateral plasma membrane, and in membranes of Golgi vesicles (Gohlke et al. 2017). The combined action of two independent but redundant endocytic recycling mechanisms, together with distinct labels for vacuolar degradation, determines the final fate of the intracellular traffic of the Chs3 protein, allowing yeast cells to regulate morphogenesis, depending on environmental constraints (Arcones et al. 2016).

Eukaryota
Fungi
Chs3 of Saccharomyces cerevisiae
4.D.1.1.13









Glycosyltransferase (Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase) of 322 aas with 2-3 C-terminal TMSs, ArnC. Catalyzes the transfer of 4-deoxy-4-formamido-L-arabinose from UDP to undecaprenyl phosphate. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (Breazeale et al. 2002).  This protein does not catalyze transport and is homologous to other enzymes in the GT2 family only in the N-terminal cytoplasmic glycosyl transferase domain. 

Bacteria
Proteobacteria
ArnC of E. coli
4.D.1.1.14









GtrB of 318 aas and 2 TMSs.  The 3.0 Å resolution crystal structure of GtrB, a glucose-specific polyisoprenyl-glycosyltransferase (PI-GT) from Synechocystis, has been reported showing a tetramer in which each protomer contributes two helices to a membrane-spanning bundle (Ardiccioni et al. 2016). The active site is 15 Å from the membrane, raising the question of how water-soluble and membrane-embedded substrates are brought into apposition for catalysis. A conserved juxtamembrane domain harbours disease mutations, which compromised activity in GtrB in vitro and in human DPM1 tested in zebrafish.  A role of this domain in shielding the polyisoprenyl-phosphate for transport to the active site has been proposed (Ardiccioni et al. 2016).

Bacteria
Cyanobacteria
GtrB of Synechocystis sp.
4.D.1.1.15









Poly-beta-1,6-N-acetyl-D-glucosamine (NAG) synthase with a 5 TMS + hydrophilic glycosyl transferase domain +4 or 5 more TMSs.  Only the middle hydrophilic glycosyltransferase domain is homologous to other members of the family.

Archaea
Candidatus Odinarchaeota
Poly-β-1,6-NAG synthase of Candidatus Odinarchaeota archaeon
4.D.1.1.16









Chitin synthase II, CHS2, of 963 aas and 7 TMSs in a 5 + 2 TMS arrangement.  It is essential for septum formation and cell division and is required for maintaining normal cell morphology.

Eukaryota
Fungi
CHS2 of Saccharomyces cerevisiae (Baker's yeast)
4.D.1.1.17









Chitin synthase I, CHS1 of 1131 aas and 7 TMSs in a 5 + 2 TMS arrangement. It is necessary for septum formation and repair, especially under certain adverse conditions.

Eukaryota
Fungi
CHS1 of Saccharomyces cerevisiae (Baker's yeast)
4.D.1.1.18









Hyaluronan synthase 2, HAS2, of 552 aas and 7 or 8 TMSs with 2 or 3 TMSs at the N-terminus and 5 TMSs at the C-terminus. It is 50% identical to HAS1 (TC# 4.D.1.1.10). It catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer and is essential to hyaluronan synthesis, a major component of most extracellular matrices. It plays a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. Autophagic degradation of HAS2 in endothelial cells provides a mechanism for the regulation of angiogenesis (Chen et al. 2020).

 

Eukaryota
Opisthokonta
NAS2 of Homo sapiens
4.D.1.2.1









Putative glycosyl transferase of 543 aas and 5 TMSs in a 2 (N-terminal) + 3 (C-terminal) TMS arrangement

Bacteria
Candidatus Saccharibacteria
GTr of Candidatus Saccharibacteria bacterium
4.D.1.2.2









Putative glycosyl transferase of 585 aas and 5 TMSs in a 2 + 3 TMS arrangement

Bacteria
Actinobacteria
Glycosyltransferase of Brevibacterium casei
4.D.1.2.3









Putative glycosyl transferase of 557 aas and 5 TMSs in a 2 + 3 TMS arrangement

Bacteria
Candidatus Saccharibacteria
Conserved membrane protein of Candidatus Saccharibacteria
4.D.1.3.1









Glycosyl transferase_GTA with a C-terminal TRP domain of 411 aas.

Bacteria
Chlamydiae/Verrucomicrobia group
GTA of Parachlamydia acanthamoebae
4.D.1.3.2









UDP glucose-lipopolysaccharide glycosyl transferase of 252 aas.

Bacteria
Proteobacteria
UDP glucose LPS glycosyl transferase of Haemophilus parasuis
4.D.1.3.3









Glycosyl transferase of 1,435 aas

Bacteria
Proteobacteria
Glycosyl transferase of Geobacter lovleyi
4.D.1.3.4









Glycosyl transferase of 1435aas, RfbC. Functions with ABC exporter, TC#3.A.1.103.3

Bacteria
Proteobacteria
RfbC of Myxococcus xanthus (Q50864)
4.D.1.3.5









Uncharacterized protein of 252 aas and 0 TMSs. Annotated as a family 2 glycosyltransferase.

Bacteria
Candidatus Beckwithbacteria
UP of  Candidatus Beckwithbacteria bacterium
4.D.1.4.1









UDP glucose:ceremide glucosyl transferase of 394 aas and 4 TMSs in a 1 + 3 TMS arrangment. May serve as a flippase (Ichikawa et al. 1996).

Eukaryota
Metazoa
Ceramide glucosyl transferase of Homo sapiens
4.D.1.4.2









Ceramide glucosyl transferase, Cgt. of 479 aas

Eukaryota
Fungi
Cgt of Ogataea parapolymorpha (Yeast) (Hansenula polymorpha)
4.D.1.4.3









Ceramide glucosyl transferase, Cgt, of 390 aas

Bacteria
Nitrospirae
Cgt of Thermodesulfovibrio yellowstonii
4.D.1.4.4









Hopinoid biosynthesis associated glycosyl transferase of 390 aas and ~5 TMSs (Schmerk et al. 2015).

Bacteria
Armatimonadetes
HpnI of Chthonomonas calidirosea