4.H.1.1.1
The 14 TMS FmtC (MprF: Multiple peptide resistance factor) protein of 840 aas, involved in methicillin and daptomycin resistance. Residues 1-550 comprise a 14TMS MFS permease domain while residues 551-840 comprise a "phosphatidylglycerol lysyl transferase" (or synthetase) domain (DUF2156), also called "lysyl cardiolipid synthase" (Oku et al. 2004; Staubitz et al. 2004; Ernst et al. 2009). FmtC may be distantly related to lysyl-tRNA synthetases (TC# 9.B.111). Similar to 2.A.1.3.44 in all these respects. Ernst et al. 2015 and Slavetinsky et al. 2012 have reported that TMSs 1-6 of FmtC of S. aureus is a flippase for lysylinated phosphatidyl glycerol, and that the entire system is a dimer.
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| Accession Number: | D1QCY9 |
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| Protein Name: | FmtC (MrpF) protein |
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| Length: | 840 |
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| Molecular Weight: | 96892.00 |
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| Species: | Staphylococcus aureus A9765 [553594] |
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| Number of TMSs: | 15 |
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| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
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| Substrate |
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1: MNQEVKNKIF SILKITFATA LFIFVAITLY RELSGINFKD TLVEFSKINR MSLVLLFIGG
61: GASLVILSMY DVILSRALKM DISLGKVLRV SYIINALNAI VGFGGFIGAG VRAMVYKNYT
121: HDKKKLVHFI SLILISMLTG LSLLSLLIVF HVFDASLILD KITWVRWVLY VVSFFLPLFI
181: IYSMVRPPDK NNRFVGLYCT LVSCVEWLAA AVVLYFCGVI VDAHVSFMSF IAIFIIAALS
241: GLVSFIPGGF GAFDLVVLLG FKTLGVPEEK VLLMLLLYRF AYYFVPVIIA LILSLFEFGT
301: SAKKYIEGSK YFIPAKDVTS FLMSYQKDII AKIPSLSLAI LVFFTSMIFF VNNLTIVYDA
361: LYDGNHLTYY ILLAIHTSAC LLLLLNVVGI YKQSRRAIIF AMISILLITV ATFFTYASYI
421: LITWLAIIFV LLIVAFRRAR RLKRPVRMRN IVAMLLFSLF ILYVNHIFIA GTLYALDIYT
481: IEMHTSVLRY YFWLTILIIA IIIGMIAWLF DYQFSKVRIS SKIEDCEEII NQYGGNYLSH
541: LIYSGDKQFF TNENKTAFLM YRYKASSLVV LGDPLGDENA FDELLEAFYN YAEYLGYDVI
601: FYQVTDQHMP LYHNFGNQFF KLGEEAIIDL TQFSTSGKKR RGFRATLNKF DELNISFEII
661: EPPFSTEFIN ELQHVSDLWL DNRQEMHFSV GEFNEEYLSK APIGVMRNEE NEVIAFCSLM
721: PTYFNDAISV DLIRWLPELD LPLMDGLYLH MLLWSKEQGY TKFNMGMATL SNVGQLHYSY
781: LRERLAGRVF EHFNGLYRFQ GLRRYKSKYN PNWEPRFLVY RKDNSLWESL SKVMRVIRHK