TCDB is operated by the Saier Lab Bioinformatics Group

8.C.10.  The Sec61-binding Cytotoxin, Ipomoeassin F (Ipomoeassin) Family 

Ipomoeassin F is a potent natural cytotoxin that inhibits growth of many tumor cell lines with single-digit nanomolar potency. Zong et al. 2019 used chemical proteomics to identify Sec61α (protein transport protein Sec61 subunit alpha isoform 1), the pore-forming subunit of the Sec61 protein translocon, as a direct binding partner of ipomoeassin F in living cells. The interaction is specific and strong enough to survive lysis conditions, enabling a biotin analogue of ipomoeassin F to pull down Sec61α from live cells, yet it is also reversible. Sec61α forms the central subunit of the ER protein translocation complex, and the binding of ipomoeassin F results in selective inhibition of protein translocation in vitro and in vivo. Ipomoeassin F thus represents a plant-derived, carbohydrate-based member of a novel structural class (Zong et al. 2019).

References associated with 8.C.10 family:

Zong, G., Z. Hu, S. O''Keefe, D. Tranter, M.J. Iannotti, L. Baron, B. Hall, K. Corfield, A.O. Paatero, M.J. Henderson, P. Roboti, J. Zhou, X. Sun, M. Govindarajan, J.M. Rohde, N. Blanchard, R. Simmonds, J. Inglese, Y. Du, C. Demangel, S. High, V.O. Paavilainen, and W.Q. Shi. (2019). Ipomoeassin F Binds Sec61α to Inhibit Protein Translocation. J. Am. Chem. Soc. 141: 8450-8461. 31059257