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The yeast retromer-dependent Vacuolar Protein Sorting (VPS) apparatus (Feyder et al. 2015). The sorting nexin (SNX) proteins, homologous to retromer-dependent VPS protein, Q92331, that normally function in endosomal membrane trafficking, are a major class of inclusion-associated host proteins in chlamydial infection (Paul et al. 2017). Some of the components of this system are homologous to those of the retromer complexes in animals (see 9.A.3; McNally and Cullen 2018).

The retromer-dependent VPS complex of Saccharomyces cerevisiae
Vps10, P32319, PEP1, VPT1, 1579 aas with two TMSs, one N-terminal, one C-terminal
Vps1, P21576, dynamin-like GTPase of 704 aas
Ypt7, P32939, VAM4 GTP binding protein/GTPase of 208 aas
Retromer constituents:
Vps35, P34110, VPT7, 944 aas
Vps29, P38759, PEP11, VPT6, 282 aas
Vps26, P40335, PEP8, VPT4, GRD6, 279 aas
Vps5, Q92331, GRD2, PEP10, 675 aas
Vps17, P32913, PEP21, 551 aas

Core of the retromer-mediated endocytic recycling complex, Vps35 (852 aas)-Vps26 (268 aas)-Vps29 (203 aas); mediates retrograde transport that is essential for parasite integrity and host infection (Sangaré et al. 2016). Signaling defect in vps26 mutants may be the result of impaired Notch signaling based on the misexpression of multiple proteins in the Notch signaling pathway in vps26 mutants (Starble and Pokrywka 2018).

Core structure of the retromer-mediated endocytic recycling complex of Toxoplasma gondii
Vacuolar protein sorting-associated protein 35, Vps35
Vacuolar protein sorting-associated protein 26, Vps26
Vacuolar protein sorting-associated protein 29, Vps29

Sortlin, SORT1 of 831 aas and 2 TMSs, N- and C-terminal (Coutinho et al. 2012). Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR; LIMP2; TC# 9.B.39.1.8). Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs) (Bogan 2012). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin (Huang et al. 2013). May also mediate transport from the endoplasmic reticulum to the Golgi (Bogan 2012).

SORT1 of Homo sapiens

The VPS10 domain-containing receptor SorCS3 of 1222 aas and 2 TMSs, also called the sortilin-related VPS10 domain-containing receptor 3. It is a type-I receptor transmembrane protein and a member of the vacuolar protein sorting 10 receptor family. Proteins of this family are defined as having a vacuolar protein sorting 10 domain at the N-terminus. members of this family play important roles as sorting agents within the cells and transport a variety of intracellular proteins between the Golgi apparatus, endosome, lysosome, secretory granules, and plasma membrane (Alfadhel et al. 2018).

SorCS3 of Homo sapiens

VPS10 domain-containing neuropeptide receptor, SorCS1, of 1168 aas and 2 putative TMSs, near the N- and C-termini of the protein.

SorCS1 of Homo sapiens