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9.A.69 The Intermembrane Phospholipid Translocase (IMPL-T) Family 

The outer membranes of gram-negative bacteria are asymmetric bilayers in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer- and inner-leaflet, respectively. This asymmetry is important for membrane integrity. In Escherichia coli, the Mla transport pathway (TC# 3.A.1.27.3) maintains this asymmetry by removing phospholipids from the outer-leaflet (Malinverni and Silhavy 2009). The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT). Nakayama and Zhang-Akiyama 2016 showed that these two proteins are components of novel transport pathways that contribute to membrane integrity. The pqiAB operon is regulated by SoxS and RpoS. The yebST operon contains pqiAB homologous genes. Nakayama and Zhang-Akiyama 2016 found a third member of the pqi operon, ymbA (pqiC). A PqiB-PqiC complex bridges the inner- and the outer-membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins. Simultaneous deletion of the pqiABC and yebST operons in a Δmla background rendered cells more sensitive to SDS/EDTA, and the SDS/EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC. The yebST operon was induced by a defect in LPS molecules. Thus, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity. Nakayama and Zhang-Akiyama 2016 thus proposed that the stress-inducible pqiABC and yebST operons encode transport pathway proteins related to the Mla transport pathway.

References associated with 9.A.69 family:

Isom, G.L., N. Coudray, M.R. MacRae, C.T. McManus, D.C. Ekiert, and G. Bhabha. (2020). LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope. Cell 181: 653-664.e19. 32359438
Malinverni, J.C. and T.J. Silhavy. (2009). An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane. Proc. Natl. Acad. Sci. USA 106: 8009-8014. 19383799
Nakayama, T. and Q.M. Zhang-Akiyama. (2016). pqiABC and yebST, putative mce operons of Escherichia coli, encode transport pathways and contribute to membrane integrity. J. Bacteriol. [Epub: Ahead of Print] 27795327