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9.B.417.  The MCFD2/LMAN1 Complex Receptor (MLM-CR) Family 

LMAN ( a mannose-specific lectin, Ergic-53; 510 aas with two TMSs, N- and C-terminal) and MCFD2 of 146 aas and one N-terminal TMS, form a complex that recognizes sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors, It may be involved in the sorting and/or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins (Nufer et al. 2003; Zhang et al. 2003). It plays a role in the secretion of coagulation factors. A 1.60 Å resolution crystal structure of the ERGIC-53(CRD)-MCFD2 complex is available showing that MCFD2, but not ERGIC-53-CRD, exhibits conformational plasticity that may be relevant to its accommodation of various polypeptide ligands (Satoh et al. 2020). The LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of alpha1-antitrypsin (Zhang et al. 2022).


References associated with 9.B.417 family:

Nufer, O., F. Kappeler, S. Guldbrandsen, and H.P. Hauri. (2003). ER export of ERGIC-53 is controlled by cooperation of targeting determinants in all three of its domains. J Cell Sci 116: 4429-4440. 13130098
Satoh, T., M. Nishio, K. Suzuki, M. Yagi-Utsumi, Y. Kamiya, T. Mizushima, and K. Kato. (2020). Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport. Acta Crystallogr F Struct Biol Commun 76: 216-221. 32356523
Zhang, B., M.A. Cunningham, W.C. Nichols, J.A. Bernat, U. Seligsohn, S.W. Pipe, J.H. McVey, U. Schulte-Overberg, N.B. de Bosch, A. Ruiz-Saez, G.C. White, E.G. Tuddenham, R.J. Kaufman, and D. Ginsburg. (2003). Bleeding due to disruption of a cargo-specific ER-to-Golgi transport complex. Nat. Genet. 34: 220-225. 12717434
Zhang, D., L. Ye, S. Hu, Q. Zhu, C. Li, and C. Zhu. (2022). Comprehensive Analysis of the Expression and Prognostic Value of LMAN2 in HER2+ Breast Cancer. J Immunol Res 2022: 7623654. 35707004
Zhang, Y., M. Zhu, C. Zheng, W. Wei, B.T. Emmer, and B. Zhang. (2022). LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin. Biochem. J. 479: 839-855. 35322856