TCDB is operated by the Saier Lab Bioinformatics Group

9.B.437.  The ESCRT-interacting Dense Granule Protein GRA64 (GRA64) Family 

GRA64 (TGME49_202620) is a transmembrane dense granule protein of 330 aas and 2 TMSs, N- and C-terminal, on the parasitophorous vacuolar membrane (PVM) of the intracellular parasite Toxoplasma gondii.  It is partially exposed to the host cell cytoplasm in both tachyzoite and bradyzoite parasitophorous vacuoles (Mayoral et al. 2022). GRA64 interacts with components of the host endosomal sorting complexes required for transport (ESCRT) (TC# 3.A.31). Δgra64 tissue cysts, enlarged vesicular structures underneath the cyst membrane, suggesting a role for GRA64 in organizing the recruitment of ESCRT proteins and subsequent intracystic vesicle formation. There seem to be very few homologs of this protein based on NCBI BLAST searchs on 7/1/2022.

References associated with 9.B.437 family:

Gardner, J.O., A.M. Leidal, T.A. Nguyen, and J. Debnath. (2023). LC3-dependent EV loading and secretion (LDELS) promotes TFRC (transferrin receptor) secretion via extracellular vesicles. Autophagy 19: 1551-1561. 36286616
Mayoral, J., R.B. Guevara, Y. Rivera-Cuevas, V. Tu, T. Tomita, J.D. Romano, L. Gunther-Cummins, S. Sidoli, I. Coppens, V.B. Carruthers, and L.M. Weiss. (2022). Dense Granule Protein GRA64 Interacts with Host Cell ESCRT Proteins during Infection. mBio e0144222. [Epub: Ahead of Print] 35730903
Naveed, M., K. Imran, A. Mushtaq, A.S. Mumtaz, H.A. Janjua, and N. Khalid. (2018). functional and tumor suppressor role of hypothetical protein PCNXL2 with regulation of the Notch signaling pathway. RSC Adv 8: 21414-21430. 35539910