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1.A.104 The Proposed Flagellar Biosynthesis Na+ Channel, FlhA (FlhA) Family 

The flagellar type III export apparatus utilizes ATP and the proton motive force (PMF) to transport flagellar proteins to the distal end of the growing flagellar structure for self-assembly. The transmembrane export gate complex is a H+-protein antiporter. Activity is greatly augmented by an associated cytoplasmic ATPase complex. Minamino et al. 2016 reported that the export gate complex can use the sodium motive force (SMF) in addition to the PMF to drive protein export. Protein export was considerably reduced in the absence of the ATPase complex and a pH gradient across the membrane, but Na+ increased it dramatically. Phenamil, a blocker of Na+ translocation, inhibited protein export. Overexpression of FlhA increased the intracellular Na+ concentration in the presence of 100 mM external NaCl but not in its absence, suggesting that FlhA acts as a Na+ channel. In wild-type cells, however, neither Na+ nor phenamil affected protein export, indicating that the Na+ channel activity of FlhA is suppressed by the ATPase complex. Minamino et al. 2016 proposed that the export gate by itself is a dual fuel engine that uses both the PMF and the SMF for protein export, and that the ATPase complex switches this dual fuel engine into a PMF-driven export machinery to become much more robust against environmental changes in external pH and Na+ concentration. Δpsi is required for efficient interaction between FliJ and FlhA to open the FlhA ion channel to conduct protons which drives flagellar protein export in a Δpsi-dependent manner (Minamino et al. 2021).

References associated with 1.A.104 family:

Minamino, T., Y. Inoue, M. Kinoshita, and K. Namba. (2020). FliK-Driven Conformational Rearrangements of FlhA and FlhB Are Required for Export Switching of the Flagellar Protein Export Apparatus. J. Bacteriol. 202:. 31712281
Minamino, T., Y.V. Morimoto, M. Kinoshita, and K. Namba. (2021). Membrane voltage-dependent activation mechanism of the bacterial flagellar protein export apparatus. Proc. Natl. Acad. Sci. USA 118:. 34035173
Minamino, T., Y.V. Morimoto, N. Hara, P.D. Aldridge, and K. Namba. (2016). The Bacterial Flagellar Type III Export Gate Complex Is a Dual Fuel Engine That Can Use Both H+ and Na+ for Flagellar Protein Export. PLoS Pathog 12: e1005495. 26943926