1.C.75.1.8
Cytopathogenic toxin, CptA, of 1991 aas and an N-terminal signal TMS. Sneathia amnii is a poorly characterized emerging pathogen that has been implicated in amnionitis and urethritis. Gentile et al. 2020 found that S. amnii damages fetal membranes, and they identified and purified the cytotoxic
exotoxin, CptA, that lyses human red blood cells and damages cells from fetal
membranes. It binds to red blood cell membranes and forms pores with a diameter of 2.0-3.0 nanometers, resulting in osmolysis. There is an association between Sneathia vaginalis and preterm birth (O'Brien et al. 2023). The Gram-negative anaerobe produces a large exotoxin, the
cytopathogenic toxin A (CptA), that forms pores in human epithelial
cells and red blood cells. In silico analysis predicts that a large amino-terminal
region of the protein is globular and separated from the
carboxy-terminal tandem repeats by a disordered region. O'Brien et al. 2023 found that a
recombinant protein consisting of the predicted structured
amino-terminal portion of CptA and devoid of the repeat region was
sufficient to permeabilize epithelial cells and red blood cells. The
repeat region was capable of binding to epithelial cells but did not
permeabilize them or lyse red blood cells. CptA is the only S. vaginalis virulence factor that has been examined mechanistically (O'Brien et al. 2023).
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| Accession Number: | AKC96038.1 |
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| Protein Name: | AKC96038.1 hypothetical protein VC03_06090 [Sneathia amnii] |
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| Length: | 1882 |
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| Molecular Weight: | |
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| Species: | Sneathia amnii [187101] |
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| Number of TMSs: | 1 |
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| Substrate |
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1: MSKFRRVLLA LFLITLVSSE VMAKTIVDTT KSNKTKVKEM NGTTVIDIDT PNSNGVSVNY
61: FKHFDVEKNG TVLNNSNSDY VTSKGLKISK NENLSSNEAK VALLKVNGTE KSALKGLLEA
121: ASGSELDVYI SNENGLVVDG LTLENIRKAV LTTGKIDDNL NITVEKGDIR ITKNGLSGSK
181: FIDLLTVKLI NEGKIKANDE VRVILGSNNN DAKVDASKLG SMYSNKITIS SPNKFLNVDT
241: HELLSKESTN IDIQGELTNK GTIASEKNVT IKAKNISNEG KVDFSKGGYE IKWITPDGKV
301: FSLEDINNKW KKKYHKSLTP SDTFLANAGK TLSGFVYDIN FTKKNVRQGA SLYDKFNAVL
361: DELDKEDKYT LLSREERQEL RNKQIDSGFV NKGDFYTTEA GGKKVLEAFA VSKTKADQAK
421: ILGENVNLEA KENVVNKDAL ILGKDKVSIK AKKFENKNTL SNEIELKDGY ENVSWRADID
481: KINHSRQYYM LKYDIDYERD LRTDSKTRKT KIAAVNKSLV SGNNVSINAE NVNLSDEKNT
541: GISLKGKNIQ VTSKNFNING QNIEASNINI TTNKANLSNA GIYSDKVSIK AKDMSILSKK
601: ETEILFDRGK ESMQKENILG SIIQSNKDID LSVDNLKLKG SIIQSKDGKV HLKGNDFNLE
661: AENISKTYNA NYDIPETFRK IKNHEKLEQA VGSFVSGKDI KIEYKKTNTK GSGLLANNIN
721: INANKVNINT ANIKHELDRR IGVDNGILGV NYNKIKKQEN MSIGSIILSK GDVSTKVDQE
781: FNLIGSKIKG KKINIEGTSI NISGKTDTTT TDTASRHLLA GDLQGSYSGG VQKNDISANV
841: EMEVYKKTKG HKVEESNNKS QIQGEDVNIN ATKKTKIEGS DVEGNNVNIH GENVEITSKK
901: TTNTEHNESE IHKVKVNASV NLGKDGISAN SNNEYSVEHK TTNSVETTEN GSNIKGKNIS
961: IIADNKVKIE GSNVEGKESV KIKGKNGVEI SSKTEVKKYE AAVQLCKLVL NAKFKEKTLG
1021: IVGTIKELVD ERDNITKYLL NIPGAIKKYK DVKKTLKENT ESIKKAIANI KANGVDPMTL
1081: GISVDLEGMI SYKPEYINAL YTSIKESTIS AKEVEISSNK DVNINGSKVK GNKVSIEAEN
1141: LNINGSNIVE KTNKTEGNVS VGGKYNIVTN ETSGSVGVSA TTSGTKKVTN AKSEITANEV
1201: NITTKKDMNV QGSKVEANSG QINVGKNLNI KHKTDKKTEY TANVNGTYNP NDNSGTVGAN
1261: LNVTHESKKN ETVIKVNNIS VNGKKNETSE TEVNVKVEGN VAVDAKKDGT TANGNVGAST
1321: KLKGDHGNGE IGLNLGGQVD KDGKLVNPKL ESHSKGQVNV GNTTINVENS ITGSKDGITT
1381: NTNASGNTNI SLDAKHAKGN VNIGFGGNLS SNEKGKIDAN GNFNTNGEVT IKGQKGNVNG
1441: EVTLAGKMDI TKGTNKPTVA SIDADGKGHV TVQGENKHIK GNVTISADGK FGADNTNGVN
1501: KLEGNANTNG TATIKXQNSI VNGEVTLNAN SNVSAGKGKK TTASVEADGK GHVTVQGENK
1561: HIKGNVTISA DGKFGADNTN GVNKLEGNAN TNGTATIKGQ NSIVNGEVTL NANSNVSAGK
1621: GKKTTASVEA DGKGHVTVQG ENKHIKGNVT ISADGKFGAD NTNGVNKLEG NANTNGTATI
1681: KGQNSIVNGE VTLNANSNVS AGKGKKTTAS VEADGRGHVT VQGENKHIKG NVTISADGKF
1741: GADNTNGVNK LEGNANTNGT ATIKGQNSIV NGEVTLNANS NVSAGKGKKT TASIDANGKG
1801: HANINVQVGN VSATANVSAQ GSVSASTNKP VKTNVTANVS KPKVTISKPK VNNVKPKKLK
1861: DKVDMKEIRR NVLSRFRRRH R