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9.A.49 The Prenylated Rab Acceptor Protein 1 (PRA1) Family 

PRA1 domain family, member 2 (PRAF2) is a 19-kDa protein with a prenylated Rab acceptor 1 (PRA1) motif and four TMSs. PRAF2 is highly expressed in the brain and serves as a candidate prognostic marker in neuroblastoma (NB). PRAF2 is related to proteins PRAF1 (PRA1, prenylin, Yip3) and PRAF3 (GTRAP3-18, JWA, Arl6-IP5), both of which are enriched in the brain and implicated in cellular transport and endo/exocytic vesicle trafficking. The distribution and localization of PRAF2 in the mature human brain  revealed that in the human cerebellum, the PRAF2 protein is strongly expressed in Purkinje cells and, more moderately, in cells of the molecular and the granular layers (Koomoa et al. 2008; ). In the cerebral cortex, hippocampus and lateral ventricles, PRAF2 can be detected in neuronal cells but not non-neuronal cells. PRAF2 is enriched in synaptic vesicles prepared from rat brains. The expression of PRAF2 in specific regions of the brain including synaptic vesicles suggest a physiological function in participatioin of multiple aspects of synaptic vesicle maturation, transport and signal transmission. Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites, suggesting that it has a functional role within the ER (Abu Irqeba and Ogilvie 2020).

References associated with 9.A.49 family:

Abu Irqeba, A. and J.M. Ogilvie. (2020). Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites. PLoS One 15: e0243075. 33259547
Da Silva, E., M.G.H. Scott, H. Enslen, and S. Marullo. (2023). Control of CCR5 Cell-Surface Targeting by the PRAF2 Gatekeeper. Int J Mol Sci 24:. 38139265
Dickison, V.M., A.M. Richmond, A. Abu Irqeba, J.G. Martak, S.C. Hoge, M.J. Brooks, M.I. Othman, R. Khanna, A.J. Mears, A.Y. Chowdhury, A. Swaroop, and J.M. Ogilvie. (2012). A role for prenylated rab acceptor 1 in vertebrate photoreceptor development. BMC Neurosci 13: 152. 23241222
Fo, C.S., C.S. Coleman, C.J. Wallick, A.L. Vine, and A.S. Bachmann. (2006). Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2). Gene 371: 154-165. 16481131
Geerts, D., C.J. Wallick, D.L. Koomoa, J. Koster, R. Versteeg, R.C. Go, and A.S. Bachmann. (2007). Expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in neuroblastoma: correlation with clinical features, cellular localization, and cerulenin-mediated apoptosis regulation. Clin Cancer Res 13: 6312-6319. 17975142
Koomoa, D.L., R.C. Go, K. Wester, and A.S. Bachmann. (2008). Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles. Neurosci Lett 436: 171-176. 18395978
Liang, Z., H. Veeraprame, N. Bayan, and G. Li. (2004). The C-terminus of prenylin is important in forming a dimer conformation necessary for endoplasmic-reticulum-to-Golgi transport. Biochem. J. 380: 43-49. 14979871
Schweneker, M., A.S. Bachmann, and K. Moelling. (2005). JM4 is a four-transmembrane protein binding to the CCR5 receptor. FEBS Lett. 579: 1751-1758. 15757671
Tanimoto, K., K. Suzuki, E. Jokitalo, N. Sakai, T. Sakaguchi, D. Tamura, G. Fujii, K. Aoki, S. Takada, R. Ishida, M. Tanabe, H. Itoh, Y. Yoneda, M. Sohda, Y. Misumi, and N. Nakamura. (2011). Characterization of YIPF3 and YIPF4, cis-Golgi Localizing Yip domain family proteins. Cell Struct Funct 36: 171-185. 21757827