TCDB is operated by the Saier Lab Bioinformatics Group

9.B.311  The 6-7 TMS TRAM/LAG (TRAM/LAG) Family 

This family is a large diverse family of proteins most frequently with about 220 - 300 aas and usually 6 or 7 TMSs, but variants occur, particularly larger homologues.  They are found in many eukaryotes including animals, plants and fungi as well as lower eukaryotes, but they do not appear to occur in prokaryotes. TRAMs are sphingolipid-binding proteins (Zimmerli et al. 2021).

The family includes members of diverse function and has been given many different names including Calfacilitin ( a modulator of Ca2+ L-type CAV1.2 channel function), CLN8, Ceremide synthase, Sphingosine N-acyl transferase, TRAM/LAG1/CLN8-like, TLC-domain-containing protein, LAG1 longevity assurance protein, Fam57A, TMM57A, Fam56, 56B, 56C, 56X1, TMM56B, TMEM56C. TMEM56X1, TMM136 and DUF887.  The name of the family used in TCDB refers to two domains in these proteins, the TRAM and the LAG domains.

References associated with 9.B.311 family:

Chen, Q., B. Denard, C.E. Lee, S. Han, J.S. Ye, and J. Ye. (2016). Inverting the Topology of a Transmembrane Protein by Regulating the Translocation of the First Transmembrane Helix. Mol. Cell 63: 567-578. 27499293
Klein, M.C., M. Lerner, D. Nguyen, S. Pfeffer, J. Dudek, F. Förster, V. Helms, S. Lang, and R. Zimmermann. (2020). TRAM1 protein may support ER protein import by modulating the phospholipid bilayer near the lateral gate of the Sec61-channel. Channels (Austin) 14: 28-44. 32013668
Stefanovic, B., L. Stefanovic, B. Schnabl, R. Bataller, and D.A. Brenner. (2004). TRAM2 protein interacts with endoplasmic reticulum Ca2+ pump Serca2b and is necessary for collagen type I synthesis. Mol. Cell Biol. 24: 1758-1768. 14749390
Wang, H., A.P. Pang, W. Wang, B. Li, C. Li, F.G. Wu, and F. Lin. (2022). Discovery of ER-localized sugar transporters for cellulase production with lac1 being essential. Biotechnol Biofuels Bioprod 15: 132. 36443855
Zimmerli, C.E., M. Allegretti, V. Rantos, S.K. Goetz, A. Obarska-Kosinska, I. Zagoriy, A. Halavatyi, G. Hummer, J. Mahamid, J. Kosinski, and M. Beck. (2021). Nuclear pores dilate and constrict in cellulo. Science 374: eabd9776. 34762489